ArticleJournal of experimental & clinical cancer research : CR2009
Tissue microarray analysis of eIF4E and its downstream effector proteins in human breast cancer.
Article in Journal of experimental & clinical cancer research : CR, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
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- An Immunohistochemical Study of Cyclin D1 Expression in Astrocytic Tumors and its Correlation with Tumor Grade.Iranian journal of pathology · 2019Article
- Estrogen receptor α dependent regulation of estrogen related receptor β and its role in cell cycle in breast cancer.BMC cancer · 2018Article
- Overexpression of eIF4F components in meningiomas and suppression of meningioma cell growth by inhibiting translation initiation.Experimental neurology · 2018Article
- Overexpression of caspase 7 is ERα dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip).Oncogenesis · 2016Article
- Expression of FOXP3, CD68, and CD20 at diagnosis in the microenvironment of classical Hodgkin lymphoma is predictive of outcome.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2013Article
- Tousled-like kinase-dependent phosphorylation of Rad9 plays a role in cell cycle progression and G2/M checkpoint exit.PloS one · 2013Article
- Immunohistochemical detection of HSP27 and hnRNP K as prognostic and predictive biomarkers for colorectal cancer.Medical oncology (Northwood, London, England) · 2012Article
- Utility of multispectral imaging in automated quantitative scoring of immunohistochemistry.Journal of clinical pathology · 2012Article
- Targeting Mnks for cancer therapy.Oncotarget · 2012Review
- Establishment of a mammary carcinoma cell line from Syrian hamsters treated with N-methyl-N-nitrosourea.Cancer letters · 2011Article
- Probabilistic analysis of gene expression measurements from heterogeneous tissues.Bioinformatics (Oxford, England) · 2010Article
- The role of MNK proteins and eIF4E phosphorylation in breast cancer cell proliferation and survival.Cancer biology & therapy · 2010Article
- Citrus auraptene suppresses cyclin D1 and significantly delays N-methyl nitrosourea induced mammary carcinogenesis in female Sprague-Dawley rats.BMC cancer · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
15 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundEukaryotic initiation factor 4E (eIF4E) is elevated in many cancers and is a prognostic indicator in breast cancer. Many pro-tumorigenic proteins are selectively translated via eIF4E, including c-Myc, cyclin D1, ornithine decarboxylase (ODC), vascular endothelial growth factor (VEGF) and Tousled-like kinase 1B (TLK1B). However, western blot analysis of these factors in human breast cancer has been limited by the availability of fresh frozen tissue and the labor-intensive nature of the multiple assays required. Our goal was to validate whether formalin-fixed, paraffin-embedded tissues arranged in a tissue microarray (TMA) format would be more efficient than the use of fresh-frozen tissue and western blot to test multiple downstream gene products.
resultsBreast tumor TMAs were stained immunohistochemically and quantitated using the ARIOL imaging system. In the TMAs, eIF4E levels correlated strongly with c-Myc, cyclin D1, TLK1B, VEGF, and ODC. Western blot comparisons of eIF4E vs. TLK1B were consistent with the immunohistochemical results. Consistent with our previous western blot results, eIF4E did not correlate with node status, ER, PR, or HER-2/neu.
conclusionWe conclude that the TMA technique yields similar results as the western blot technique and can be more efficient and thorough in the evaluation of several products downstream of eIF4E.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.