ArticleGenome biology2009
Insight into the molecular requirements for pathogenicity of Fusarium oxysporum f. sp. lycopersici through large-scale insertional mutagenesis.
Article in Genome biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 135 citations in OpenAlex.
- The Effector SIX8 Contributes to Virulence of Fusarium oxysporum f. sp. lactucae Race 4 on Lettuce.Molecular plant pathology · 2026Article
- Asparagus Decline and Replant Problem: Autotoxicity, Autotoxic Substances, and Their Biological Functions.Biology · 2026Review
- ΔFW-NPS6-Dependent Transcriptome Profiling Reveals Putative Pathogenicity Genes inInternational journal of molecular sciences · 2026Article
- FoTeRs: a novel family of telomere-associated retrotransposons in Fusarium oxysporum.Mobile DNA · 2025Article
- Divergent ECC1 effector homologs modulate host-specific virulence in cucurbit-infectingFrontiers in cellular and infection microbiology · 2025Article
- Characterization and organization of telomeric-linked helicase (tlh) gene families in Fusarium oxysporum.Current genetics · 2024Article
- The Ubiquitous Wilt-Inducing PathogenPathogens (Basel, Switzerland) · 2024Review
- Zinc finger transcription factor ZFP1 is associated with growth, conidiation, osmoregulation, and virulence in the Polygonatum kingianum pathogen Fusarium oxysporum.Scientific reports · 2024Article
- Article
- Mining theJournal of fungi (Basel, Switzerland) · 2023Article
- Fusarium oxysporum f. sp.mBio · 2023Article
- Histone H3K27 Methylation Perturbs Transcriptional Robustness and Underpins Dispensability of Highly Conserved Genes in Fungi.Molecular biology and evolution · 2022Article
- A primary cell wall cellulose-dependent defense mechanism against vascular pathogens revealed by time-resolved dual transcriptomics.BMC biology · 2021Article
- Current progress on pathogenicity-related transcription factors in Fusarium oxysporum.Molecular plant pathology · 2021Review
- Secreted in Xylem Genes: Drivers of Host Adaptation inFrontiers in plant science · 2021Review
- The Destructive Fungal PathogenPathogens (Basel, Switzerland) · 2020Review
- Related mobile pathogenicity chromosomes in Fusarium oxysporum determine host range on cucurbits.Molecular plant pathology · 2020Article
- Pathogenomics and Management ofPathogens (Basel, Switzerland) · 2020Review
- Pathogenicity and Virulence Factors ofMicroorganisms · 2020Review
- Calcineurin Regulates Conidiation, Chlamydospore Formation and Virulence inFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFusarium oxysporum f. sp. lycopersici is the causal agent of vascular wilt disease in tomato. In order to gain more insight into the molecular processes in F. oxysporum necessary for pathogenesis and to uncover the genes involved, we used Agrobacterium-mediated insertional mutagenesis to generate 10,290 transformants and screened the transformants for loss or reduction of pathogenicity.
resultsThis led to the identification of 106 pathogenicity mutants. Southern analysis revealed that the average T-DNA insertion is 1.4 and that 66% of the mutants carry a single T-DNA. Using TAIL-PCR, chromosomal T-DNA flanking regions were isolated and 111 potential pathogenicity genes were identified.
conclusionsFunctional categorization of the potential pathogenicity genes indicates that certain cellular processes, such as amino acid and lipid metabolism, cell wall remodeling, protein translocation and protein degradation, seem to be important for full pathogenicity of F. oxysporum. Several known pathogenicity genes were identified, such as those encoding chitin synthase V, developmental regulator FlbA and phosphomannose isomerase. In addition, complementation and gene knock-out experiments confirmed that a glycosylphosphatidylinositol-anchored protein, thought to be involved in cell wall integrity, a transcriptional regulator, a protein with unknown function and peroxisome biogenesis are required for full pathogenicity of F. oxysporum.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.