ArticleBritish journal of pharmacology2009
Do gene polymorphisms alone or in combination affect the function of human beta3-adrenoceptors?
Article in British journal of pharmacology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Chronic mirabegron treatment increases human brown fat, HDL cholesterol, and insulin sensitivity.The Journal of clinical investigation · 2020Trial
- Therapeutic Potential of the β3-Adrenergic Receptor and Its Ligands in Cardiovascular Diseases.International journal of molecular sciences · 2025Review
- Adrenoceptors: Receptors, Ligands and Their Clinical Uses, Molecular Pharmacology and Assays.Handbook of experimental pharmacology · 2024Review
- Effect of β3-adrenergic receptor gene polymorphism and lifestyle on overweight Japanese rural residents: A cross-sectional study.Obesity science & practice · 2022Article
- β3-Adrenergic receptors regulate human brown/beige adipocyte lipolysis and thermogenesis.JCI insight · 2021Article
- Functional Characterization of the Obesity-Linked Variant of the βInternational journal of molecular sciences · 2021Article
- The Regulator of G Protein Signaling Homologous Domain of G Protein-Coupled Receptor Kinase 2 Mediates Short-Term Desensitization of β3-Adrenergic Receptor.Frontiers in pharmacology · 2020Article
- Agonist-induced desensitisation of βBritish journal of pharmacology · 2019Review
- Desensitization of cAMP Accumulation via Human β3-Adrenoceptors Expressed in Human Embryonic Kidney Cells by Full, Partial, and Biased Agonists.Frontiers in pharmacology · 2019Article
- Pharmacokinetic Parameters and Over-Responsiveness of Iranian Population to Propranolol.Advanced pharmaceutical bulletin · 2017Article
- How β3 -adrenoceptor-selective is mirabegron?British journal of pharmacology · 2016Article
- What is the role for biomarkers for lower urinary tract disorders? ICI-RS 2013.Neurourology and urodynamics · 2014Article
- Do β-adrenoceptor agonists induce homologous or heterologous desensitization in rat urinary bladder?Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
- Agonist-induced desensitization of human β3-adrenoceptors expressed in human embryonic kidney cells.Naunyn-Schmiedeberg's archives of pharmacology · 2013Article
- Pharmacological profile of β3-adrenoceptor agonists in clinical development for the treatment of overactive bladder syndrome.Naunyn-Schmiedeberg's archives of pharmacology · 2013Article
- Functional investigation of β-adrenoceptors in human isolated detrusor focusing on the novel selective β3-adrenoceptor agonist KUC-7322.Naunyn-Schmiedeberg's archives of pharmacology · 2012Article
- Beta-3 adrenoceptors as new therapeutic targets for cardiovascular pathologies.Current heart failure reports · 2011Review
- Are there functional β₃-adrenoceptors in the human heart?British journal of pharmacology · 2011Article
- {beta}-Adrenoceptor gene variation and intermediate physiological traits: prediction of distant phenotype.Experimental physiology · 2010Review
- Lack of effect of the alpha2C-adrenoceptor Del322-325 polymorphism on inhibition of cyclic AMP production in HEK293 cells.British journal of pharmacology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposebeta(3)-Adrenoceptors mediate many important physiological functions, for example, in the urinary bladder. The corresponding gene is polymorphic, and the W64R (Trp64Arg) single nucleotide polymorphism has been associated with disease states such as obesity, type 2 diabetes and bladder dysfunction. While these clinical data suggest that the 64R variant is hypofunctional, previous in vitro studies in which this variant was generated by site-directed mutagenesis and subsequent transfection have not consistently confirmed this. EXPERIMENTAL APPROACH: We transfected the wild-type human beta(3)-adrenoceptor and the 64R variant and also the more recently discovered 265M and 306F variants as well as 64R/265M and 64R/306F double mutants into human embryonic kidney cells and selected clones expressing the receptors at a density of about 100 fmol mg protein(-1). Receptor activation was measured by cAMP accumulation and ligand affinity by radioligand binding. Desensitisation was assessed as alterations of cAMP responses after prolonged agonist treatment. KEY
resultsNeither mutated receptor exhibited alterations in efficacy or potency for cAMP accumulation for any of five agonists (isoprenaline, noradrenaline, YM 178, FK 4664, CGP 12 177). In competition binding studies, the mutations did not affect the ability of any agonist to bind to the receptor. Wild-type receptors and the 64R variant exhibited similar isoprenaline-induced functional desensitization during a 24 h treatment. CONCLUSIONS AND IMPLICATIONS: None of the polymorphisms tested here significantly altered the interaction of isoprenaline, noradrenaline, YM 178, FK 4664 or CGP 12 177 with the human beta(3)-adrenoceptor when expressed at near physiological levels in a human cell line.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.