ArticleInflammatory bowel diseases2009
Gene expression patterns in experimental colitis in IL-10-deficient mice.
Article in Inflammatory bowel diseases, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Quality of methods reporting in animal models of colitis.Inflammatory bowel diseases · 2015Pooled it
- The TLR9 Agonist Cobitolimod Induces IL10-Producing Wound Healing Macrophages and Regulatory T Cells in Ulcerative Colitis.Journal of Crohn's & colitis · 2020Trial
- Inhibition of IEC-6 Cell Proliferation and the Mechanism of Ulcerative Colitis in C57BL/6 Mice by Dandelion Root Polysaccharides.Foods (Basel, Switzerland) · 2023Article
- MTA1 aggravates experimental colitis in mice by promoting transcription factor HIF1A and up-regulating AQP4 expression.Cell death discovery · 2022Article
- Exploration of the Potential Link, Hub Genes, and Potential Drugs for Coronavirus Disease 2019 and Lung Cancer Based on Bioinformatics Analysis.Journal of oncology · 2022Article
- Cross-species high-resolution transcriptome profiling suggests biomarkers and therapeutic targets for ulcerative colitis.Frontiers in molecular biosciences · 2022Article
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- Extracellular vesicles produced by the human gut commensal bacteriumFrontiers in microbiology · 2022Article
- Tryptophan-derived serotonin-kynurenine balance in immune activation and intestinal inflammation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Review
- Aquaporins in Immune Cells and Inflammation: New Targets for Drug Development.International journal of molecular sciences · 2021Review
- Evaluation of the Effects of DifferentJournal of immunology research · 2021Article
- Epithelial RABGEF1 deficiency promotes intestinal inflammation by dysregulating intrinsic MYD88-dependent innate signaling.Mucosal immunology · 2020Article
- Integrated omics profiling of dextran sodium sulfate-induced colitic mice supplemented with Wolfberry (NPJ science of food · 2020Article
- Article
- Identification of novel mRNAs and lncRNAs associated with mouse experimental colitis and human inflammatory bowel disease.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
- Environmental Factors Modify the Severity of Acute DSS Colitis in Caspase-11-Deficient Mice.Inflammatory bowel diseases · 2018Article
- Intergenerational transfer of antibiotic-perturbed microbiota enhances colitis in susceptible mice.Nature microbiology · 2018Article
- Lactobacilli with superoxide dismutase-like or catalase activity are more effective in alleviating inflammation in an inflammatory bowel disease mouse model.Drug design, development and therapy · 2018Article
- New Perspectives on the Potential Role of Aquaporins (AQPs) in the Physiology of Inflammation.Frontiers in physiology · 2018Review
- Hypoxia and Mucosal Inflammation.Annual review of pathology · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundWhile others have described gene expression patterns in humans with inflammatory bowel diseases and animals with chemically induced colitis, a genome-wide comparison of gene expression in genetically susceptible animals that develop spontaneous colitis has not been reported.
methodsWe used microarray technology to compare gene expression profiles in cecal specimens from specific pathogen-free IL10-deficient (IL10-/-) mice with colitis and normal wildtype (WT) mice. RNA isolated from ceca of IL10-/- and WT mice was subjected to microarray analysis. The results were confirmed by real-time polymerase chain reaction (PCR) and immunofluorescence microscopy of selected molecules. Expression of the selected genes in dextran sodium sulfate (DSS)-treated mice with colitis and epithelial cell lines activated with pathophysiologic stimuli was measured by real-time PCR.
resultsHistological inflammation of the colon and IL-12/23p40 secretion from intestinal explants were greater in IL10-/- and DSS-treated mice versus WT and untreated mice. Microarray analysis demonstrated >10-fold induction of the following molecules in the ceca of IL10-/- mice: mitochondrial ribosomal protein-L33, aquaporin-4, indoleamine-pyrrole-2,3-dioxygenase, and MHC class II with 63, 25, 20, and 12-fold increases, respectively. Cytochrome-P450, pancreatic lipase-related protein-2, and transthyretin were downregulated in IL10-/- mice. MHC II was increased throughout the colon, and aquaporin-4 was increased in the basolateral aspect of cecal epithelial cells. MHC II mRNA was increased in epithelial cells treated with IFN-gamma, but not TNF or Toll-like receptor ligands.
conclusionsAlthough most upregulated genes in experimental colitis are immune-related, aquaporin-4 and mitochondrial ribosomal protein-L33, which have not been previously associated with inflammation, were most highly upregulated.
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Registered trials
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