ArticleGenesis (New York, N.Y. : 2000)2009
Characterization of a Dazl-GFP germ cell-specific reporter.
Article in Genesis (New York, N.Y. : 2000), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Comparative evaluation of germ cell-specific promoters identifies robust drivers of transgene expression during mouse spermatogenesis.Molecular biology reports · 2026Article
- Human testis-specific TTRP regulates circadian clock as an evolutionarily young natural antisense transcript of PER2.Nature communications · 2026Article
- Gene therapy for non-obstructive azoospermia: therapeutic avenues and translational pathway to clinical application.Frontiers in genome editing · 2026Review
- Cre Deleter Mouse Lines for Conditional Knockout of Genes in Testis Cell Types.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Proper timing of a quiescence period in precursor prospermatogonia is required for stem cell pool establishment in the male germline.Development (Cambridge, England) · 2021Article
- DAZL is a master translational regulator of murine spermatogenesis.National science review · 2019Article
- Regulation of GDNF expression in Sertoli cells.Reproduction (Cambridge, England) · 2019Review
- Advanced immunostaining approaches to study early male germ cell development.Stem cell research · 2018Article
- Long Evans rat spermatogonial lines are effective germline vectors for transgenic rat production.Transgenic research · 2017Article
- The NOTCH Ligand JAG1 Regulates GDNF Expression in Sertoli Cells.Stem cells and development · 2017Article
- DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis.Biology of reproduction · 2017Article
- Imiquimod-induced CCR9 Ameliorates murine TNBS Colitis.Fukushima journal of medical science · 2016Article
- A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways.Scientific reports · 2016Article
- Article
- Identification of a β-galactosidase transgene that provides a live-cell marker of transcriptional activity in growing oocytes and embryos.Molecular human reproduction · 2015Article
- DAZL limits pluripotency, differentiation, and apoptosis in developing primordial germ cells.Stem cell reports · 2014Article
- Aromatase-null mice expressing enhanced green fluorescent protein in germ cells provide a model system to assess estrogen-dependent ovulatory responses.Transgenic research · 2014Article
- Strategies for enrichment and selection of stem cell-derived tissue precursors.Stem cell research & therapy · 2012Review
- Isolation of undifferentiated and early differentiating type A spermatogonia from Pou5f1-GFP reporter mice.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Implication of DNA demethylation and bivalent histone modification for selective gene regulation in mouse primordial germ cells.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
Abstract
In this study, we characterized the promoter activity of a 1.7 kb sequence in the 5' flanking region of the mouse Deleted in Azoospermia-Like (Dazl) gene. We found the 1.7 kb sequence sufficient to drive robust germ cell-specific expression of green fluorescent protein (GFP) in adult mouse testis and lower levels of expression in adult ovary and in fetal and newborn gonads of both sexes. This expression pattern was confirmed in two independently-derived transgenic mouse lines. In adult testis, Dazl-GFP exhibited a developmentally-regulated, stage-specific expression pattern during spermatogenesis. GFP was highly expressed in spermatocyte stages, with strongest expression in pachytene spermatocytes. Weaker expression was observed in round and elongating spermatids, as well as spermatogonial cells. In the fetal gonad, GFP transcript was detected by e12.5 in both sexes; however, GFP fluorescence was only detected during later embryonic stages. In addition, we produced mouse embryonic stem cell (ESC) lines harboring the Dazl-GFP reporter and used this reporter to isolate putative germ cell populations derived from mouse ESCs following embryoid body differentiation and fluorescence activated cell sorting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.