ArticleEuropean biophysics journal : EBJ2009
Concentration dependent effect of GsMTx4 on mechanosensitive channels of small conductance in E. coli spheroplasts.
Article in European biophysics journal : EBJ, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Prokaryotic mechanosensitive channels mediate copper influx.Protein science : a publication of the Protein Society · 2025Article
- Involvement of MID1-COMPLEMENTING ACTIVITY 1 encoding a mechanosensitive ion channel in prehaustorium development of the stem parasitic plant Cuscuta campestris.Plant & cell physiology · 2025Article
- Approaches for the modulation of mechanosensitive MscL channel pores.Frontiers in chemistry · 2023Review
- Roles of Bacterial Mechanosensitive Channels in Infection and Antibiotic Susceptibility.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Mechanical and chemical activation of GPR68 probed with a genetically encoded fluorescent reporter.Journal of cell science · 2021Article
- Recent advances in cochlear hair cell nanophysiology: subcellular compartmentalization of electrical signaling in compact sensory cells.Faculty reviews · 2020Review
- Phosphoinositol-4,5-Bisphosphate Regulates Auditory Hair-Cell Mechanotransduction-Channel Pore Properties and Fast Adaptation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- GsMTx4: Mechanism of Inhibiting Mechanosensitive Ion Channels.Biophysical journal · 2017Article
- Review
- Effects of Lys to Glu mutations in GsMTx4 on membrane binding, peptide orientation, and self-association propensity, as analyzed by molecular dynamics simulations.Biochimica et biophysica acta · 2015Article
- Patch clamp characterization of the effect of cardiolipin on MscS of E. coli.European biophysics journal : EBJ · 2015Article
- Ion channels contribute to the regulation of cell sheet forces during Drosophila dorsal closure.Development (Cambridge, England) · 2014Article
- Article
- A venom-derived neurotoxin, CsTx-1, from the spider Cupiennius salei exhibits cytolytic activities.The Journal of biological chemistry · 2012Article
- Molecular force transduction by ion channels: diversity and unifying principles.Journal of cell science · 2012Review
- Effects of GsMTx4 on bacterial mechanosensitive channels in inside-out patches from giant spheroplasts.Biophysical journal · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spider peptide GsMTx4, at saturating concentration of 5 muM, is an effective and specific inhibitor for stretch-activated mechanosensitive (MS) channels found in a variety of eukaryotic cells. Although the structure of the peptide has been solved, the mode of action remains to be determined. Because of its amphipathic structure, the peptide is proposed to interact with lipids at the boundaries of the MS channel proteins. In addition, GsMTx4 has antimicrobial effects, inhibiting growth of several species of bacteria in the range of 5-64 microM. Previous studies on prokaryotic MS channels, which serve as model systems to explore the principle of MS channel gating, have shown that various amphipathic compounds acting at the protein-lipid interface affect MS channel gating. We have therefore analyzed the effect of different concentrations of extracellular GsMTx4 on MS channels of small conductance, MscS and MscK, in the cytoplasmic membrane of wild-type E. coli spheroplasts using the patch-clamp technique. Our study shows that the peptide GsMTx4 exhibits a biphasic response in which peptide concentration determines inhibition or potentiation of activity in prokaryotic MS channels. At low peptide concentrations of 2 and 4 microM the gating of the prokaryotic MS channels was hampered, manifested by a decrease in pressure sensitivity. In contrast, application of peptide at concentrations of 12 and 20 microM facilitated prokaryotic MS channel opening by increasing the pressure sensitivity.
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