ArticleEuropean biophysics journal : EBJ2010
The role of protons in fast and slow gating of the Torpedo chloride channel ClC-0.
Article in European biophysics journal : EBJ, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Computational Investigation of Mechanisms for pH Modulation of Human Chloride Channels.Molecules (Basel, Switzerland) · 2023Article
- Dynamical model of the CLC-2 ion channel reveals conformational changes associated with selectivity-filter gating.PLoS computational biology · 2020Article
- Proton-dependent inhibition, inverted voltage activation, and slow gating of CLC-0 Chloride Channel.PloS one · 2020Article
- Energetics and mechanism of anion permeation across formate-nitrite transporters.Scientific reports · 2017Article
- Ion Channels in Native Chloroplast Membranes: Challenges and Potential for Direct Patch-Clamp Studies.Frontiers in physiology · 2015Review
- GlialCAM, a CLC-2 Cl(-) channel subunit, activates the slow gate of CLC chloride channels.Biophysical journal · 2014Article
- A single point mutation reveals gating of the human ClC-5 Cl-/H+ antiporter.The Journal of physiology · 2013Article
- Alkaline pH block of CLC-K kidney chloride channels mediated by a pore lysine residue.Biophysical journal · 2013Article
- A thin line between channels and pumps.Biophysical journal · 2013Article
- On the mechanism of gating charge movement of ClC-5, a human Cl(-)/H(+) antiporter.Biophysical journal · 2012Article
- Mechanism of proton/substrate coupling in the heptahelical lysosomal transporter cystinosin.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A regulatory calcium-binding site at the subunit interface of CLC-K kidney chloride channels.The Journal of general physiology · 2010Article
- Antiport mechanism for Cl(-)/H(+) in ClC-ec1 from normal-mode analysis.Biophysical journal · 2010Article
- Chloride channels: often enigmatic, rarely predictable.Annual review of physiology · 2010Review
- Molecular Pharmacology of Kidney and Inner Ear CLC-K Chloride Channels.Frontiers in pharmacology · 2010Article
- It's the proton also in ClC-2.The Journal of physiology · 2009Article
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2 authors.
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Abstract
Transmembrane proton transport is of fundamental importance for life. The list of H(+) transporting proteins has been recently expanded with the discovery that some members of the CLC gene family are stoichiometrically coupled Cl(-)/H(+) antiporters. Other CLC proteins are instead passive Cl(-) selective anion channels. The gating of these CLC channels is, however, strongly regulated by pH, likely reflecting the evolutionary relationship with CLC Cl(-)/H(+) antiporters. The role of protons in the gating of the model Torpedo channel ClC-0 is best understood. ClC-0 is a homodimer with separate pores in each subunit. Each protopore can be opened and closed independently from the other pore by a "fast gate". A common, slow gate acts on both pores simultaneously. The opening of the fast gate is controlled by a critical glutamate (E166), whose protonation state determines the fast gate's pH dependence. Extracellular protons likely can arrive directly at E166. In contrast, protonation of E166 from the inside has been proposed to be mediated by the dissociation of an intrapore water molecule. The OH(-) anion resulting from the water dissociation is stabilized in one of the anion binding sites of the channel, competing with intracellular Cl(-) ions. The pH dependence of the slow gate is less well understood. It has been shown that proton translocation drives irreversible gating transitions associated with the slow gate. However, the relationship of the fast gate's pH dependence on the proton translocation and the molecular basis of the slow gate remain to be discovered.
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