ArticleNature protocols2009
Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
64 citing papers in PubMed, 172 citations in OpenAlex.
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- Enhanced RNA Preservation in Mouse Brain Tissue: A Strategy Combining Cardiac Perfusion with Hypersaline Immersion.ACS omega · 2025Article
- Identifying potential miRNA-disease associations through an accurate matrix completion approach.Briefings in bioinformatics · 2025Article
- Non-coding RNAs, a double-edged sword in breast cancer prognosis.Cancer cell international · 2025Review
- Insights into non-coding RNAS: biogenesis, function and their potential regulatory roles in acute kidney disease and chronic kidney disease.Molecular and cellular biochemistry · 2025Review
- Optimising in-cell NMR acquisition for nucleic acids.Journal of biomolecular NMR · 2024Article
- In situ imaging of intracellular miRNAs in tumour cells by branched hybridisation chain reaction.Cell proliferation · 2024Article
- CRISPR screening uncovers nucleolar RPL22 as a heterochromatin destabilizer and senescence driver.Nucleic acids research · 2024Article
- The novel pre-rRNA detection workflow "Riboprobing" allows simple identification of undescribed RNA species.RNA (New York, N.Y.) · 2024Article
- σeLife · 2023Article
- Heliangin acts as a covalent ligand of RPS2 that disrupts pre-rRNA metabolic processes inActa pharmaceutica Sinica. B · 2023Article
- Sample Preparation and Differential Gene Expression Analysis of Human Cancer Cell Lines by RNA Sequencing.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Modified Northern blot protocol for easy detection of mRNAs in total RNA using radiolabeled probes.BMC genomics · 2022Article
- Annealed ZnO/AlNanomaterials (Basel, Switzerland) · 2021Article
- Approaches to Identify and Characterise the Post-Transcriptional Roles of lncRNAs in Cancer.Non-coding RNA · 2021Review
- In Vitro and In Vivo Models for Evaluating the Oral Toxicity of Nanomedicines.Nanomaterials (Basel, Switzerland) · 2020Review
- Review
- Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple.Plant biotechnology journal · 2020Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Investigation of gene expression significantly contributes to our knowledge of the regulation and function of genes in many areas of biology. In this protocol, we describe how northern blot analysis is used to identify gene expression patterns at the RNA level in human cancer cells as well as in cancerous and normal tissues. RNA molecules are separated by gel electrophoresis and are subsequently transferred to a porous membrane by capillary action. Specific sequences in the RNA are detected on the membrane by molecular hybridization with radiolabeled nucleic acid probes. Despite the development of newer methods, such as real-time PCR, nuclease protection assays and microarrays, northern blot analysis is still a standard technique used in the detection and quantification of mRNA levels because it allows a direct comparison of the mRNA abundance between samples on a single membrane. This entire northern blotting protocol takes approximately 4 d to complete.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.