ArticleBiochimica et biophysica acta2009
Expression of phospholipases A2 in primary human lung macrophages: role of cytosolic phospholipase A2-alpha in arachidonic acid release and platelet activating factor synthesis.
Article in Biochimica et biophysica acta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Low molecular weight hyaluronan activates cytosolic phospholipase A2α and eicosanoid production in monocytes and macrophages.The Journal of biological chemistry · 2014Trial
- The Hypertensive Effect of Amphotericin B-Containing Liposomes (Abelcet) in Mice: Dissecting the Roles of C3a and C5a Anaphylatoxins, Macrophages and Thromboxane.Biomedicines · 2022Article
- Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary.Molecules (Basel, Switzerland) · 2022Article
- The interaction of secreted phospholipase A2-IIA with the microbiota alters its lipidome and promotes inflammation.JCI insight · 2022Article
- Choline chloride attenuates the allergic airway disease by inhibiting the lysophosphatidylcholine induced response in mouse model.Current research in pharmacology and drug discovery · 2022Article
- Communication between human macrophages and epithelial cancer cell lines dictates lipid mediator biosynthesis.Cellular and molecular life sciences : CMLS · 2020Article
- Secreted PLA2 group X orchestrates innate and adaptive immune responses to inhaled allergen.JCI insight · 2017Article
- Lysophosphatidylcholine plays critical role in allergic airway disease manifestation.Scientific reports · 2016Article
- An in vitro alveolar macrophage assay for predicting the short-term inhalation toxicity of nanomaterials.Journal of nanobiotechnology · 2016Article
- In vitro anti-Plasmodium falciparum properties of the full set of human secreted phospholipases A2.Infection and immunity · 2015Article
- Epidermal growth factor induces platelet-activating factor production through receptors transactivation and cytosolic phospholipase A2 in ovarian cancer cells.Journal of ovarian research · 2014Article
- The monocytic population in chronic lymphocytic leukemia shows altered composition and deregulation of genes involved in phagocytosis and inflammation.Haematologica · 2013Article
- Phospholipase A2 in experimental allergic bronchitis: a lesson from mouse and rat models.PloS one · 2013Article
- The involvement of phospholipases A2 in asthma and chronic obstructive pulmonary disease.Mediators of inflammation · 2013Review
- Lung mast cells are a source of secreted phospholipases A2.The Journal of allergy and clinical immunology · 2009Article
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Authors and funding
11 authors at 7 institutions in 3 countries.
Funding
Abstract
Macrophages are a major source of lipid mediators in the human lung. Expression and contribution of cytosolic (cPLA(2)) and secreted phospholipases A(2) (sPLA(2)) to the generation of lipid mediators in human macrophages are unclear. We investigated the expression and role of different PLA(2)s in the production of lipid mediators in primary human lung macrophages. Macrophages express the alpha, but not the zeta isoform of group IV and group VIA cPLA(2) (iPLA(2)). Two structurally-divergent inhibitors of group IV cPLA(2) completely block arachidonic acid release by macrophages in response to non-physiological (Ca(2+) ionophores and phorbol esters) and physiological agonists (lipopolysaccharide and Mycobacterium protein derivative). These inhibitors also reduce by 70% the synthesis of platelet-activating factor by activated macrophages. Among the full set of human sPLA(2)s, macrophages express group IIA, IID, IIE, IIF, V, X and XIIA, but not group IB and III enzymes. Me-Indoxam, a potent and cell impermeable inhibitor of several sPLA(2)s, has no effect on arachidonate release or platelet-activating factor production. Agonist-induced exocytosis is not influenced by cPLA(2) inhibitors at concentrations that block arachidonic acid release. Our results indicate that human macrophages express cPLA(2)-alpha, iPLA(2) and several sPLA(2)s. Cytosolic PLA(2)-alpha is the major enzyme responsible for lipid mediator production in human macrophages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.