ArticleBiomedical microdevices2009
Sample preparation module for bacterial lysis and isolation of DNA from human urine.
Article in Biomedical microdevices, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 51 citations in OpenAlex.
- Sample preparation and detection methods in point-of-care devices towards future at-home testing.Lab on a chip · 2024Review
- MC-PRPA-HLFIA Cascade Detection System for Point-of-Care Testing Pan-Drug-Resistant Genes in Urinary Tract Infection Samples.International journal of molecular sciences · 2023Article
- An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples.Sensors (Basel, Switzerland) · 2021Review
- Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases.Biosensors & bioelectronics · 2020Review
- A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples.Biomedical microdevices · 2018Article
- DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering.Journal of visualized experiments : JoVE · 2017Article
- Simple and rapid sample preparation system for the molecular detection of antibiotic resistant pathogens in human urine.Biomedical microdevices · 2016Article
- Paper-based molecular diagnostic forRSC advances · 2014Article
- Article
- A novel surface modification technique for forming porous polymer monoliths in poly(dimethylsiloxane).Biomicrofluidics · 2012Article
- An integrated disposable device for DNA extraction and helicase dependent amplification.Biomedical microdevices · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Silica impregnated polymer monolithic columns may provide a simple method for lysing and extracting DNA from bacteria inside of microfluidic chips. Here we use Escherichia coli as a test organism for a point of care thermoplastic microfluidic module designed to take in a urine sample, mix it with lysis buffer, and perform a hybrid chemical/mechanical lysis and solid phase extraction of nucleic acids from the sample. To demonstrate proof-of-concept, we doped human hematuric urine samples with E. coli at concentrations ranging from 10(1)-10(5) colony-forming units/mL (CFU/mL) to simulate patient samples. We then performed on-chip lysis and DNA extraction. The bacterial DNA was amplified using real-time PCR demonstrating lysis and isolation down to 10(1) CFU/mL. Results were comparable to a commercial kit at higher concentrations and performed better at recovering DNA at lower concentrations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.