ArticleParasitology research2009
Proteins of the Plasmodium falciparum two transmembrane Maurer's cleft protein family, PfMC-2TM, and the 130 kDa Maurer's cleft protein define different domains of the infected erythrocyte intramembranous network.
Article in Parasitology research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Variable Surface Antigens ofProtein and peptide letters · 2024Review
- Molecular Players at the Sorting Stations of Malaria ParasiteCurrent protein & peptide science · 2024Review
- Vivax Malaria and the Potential Role of the Subtelomeric MultigeneMicroorganisms · 2022Review
- Differential Trafficking and Expression of PIR Proteins in Acute and ChronicFrontiers in cellular and infection microbiology · 2022Article
- Trafficking and Association ofPathogens (Basel, Switzerland) · 2021Article
- '2TM proteins': an antigenically diverse superfamily with variable functions and export pathways.PeerJ · 2018Article
- Article
- A comparative study of the localization and membrane topology of members of the RIFIN, STEVOR and PfMC-2TM protein families in Plasmodium falciparum-infected erythrocytes.Malaria journal · 2015Article
- HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins.Journal of visualized experiments : JoVE · 2015Article
- In vitro antiplasmodial activity of cepharanthine.Malaria journal · 2014Article
- Maurer's clefts, the enigma of Plasmodium falciparum.Proceedings of the National Academy of Sciences of the United States of America · 2013Review
- Variant surface antigens of malaria parasites: functional and evolutionary insights from comparative gene family classification and analysis.BMC genomics · 2013Article
- In vitro human cell-free expression system for synthesis of malaria proteins.Parasitology research · 2012Article
- Plasmodium falciparum infection-induced changes in erythrocyte membrane proteins.Parasitology research · 2012Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmodium falciparum Maurer's clefts participate in the transport of macromolecules within the cytoplasm, including the transport of virulence proteins to the erythrocyte membrane surface. We identified a family of genes PfMC-2TM encoding transmembrane proteins located within the intramembranous network of the infected erythrocyte using monoclonal antibody SP1C1. The distribution of the PfMC-2TM protein family within domains of the network was investigated by colocalization and confocal microscopy studies using monoclonal antibody SP1C1 specific for PFMC-2TM and monoclonal antibody SP1A6 specific for the130 kDa Maurer's cleft protein. Peptide-specific antibodies were prepared against six peptides from different domains of PfMC-2TM and used with the Mabs, as well as known antibodies specific to Maurer's clefts proteins (ring-expressed protein and membrane-associated histidine-rich protein 1), the erythrocyte membrane protein 1 (PfEMP-1), and serine-rich antigen in colocalization studies. We show that PfMC-2TM is located in the Maurer's clefts throughout the intracellular blood stage, and immunoelectron microscopy shows domains of PfMC-2TM localized in the parasitophorous vacuole and parasitophorous vacuole membrane. The distribution of the 130 kDa Maurer's cleft protein changes from within the parasite to the clefts during intracellular development as the parasite matures from young trophozoite to segmented schizont.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.