ArticleParasitology research2009
Validation of a Western Blot for the detection of anti-Trichinella spp. antibodies in domestic pigs.
Article in Parasitology research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Validation of an optimized in-house enzyme-linked immunosorbent assay for enhanced detection ofFood and waterborne parasitology · 2026Article
- Sero-epidemiological investigation on Toxoplasma gondii infection in Apennine wolf (Canis lupus italicus) and wild boar (Sus scrofa) in Italy.BMC veterinary research · 2024Article
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- A review of testing and assurance methods forFood and waterborne parasitology · 2021Article
- International Commission on Trichinellosis: Recommendations on the use of serological tests for the detection ofFood and waterborne parasitology · 2019Review
- Detection and Evaluation of Antibody Response to a Baylisascaris-Specific Antigen in Rodent Hosts with the Use of Western Blotting and Elisa.The Journal of parasitology · 2018Article
- Serological tools for detection ofOne health (Amsterdam, Netherlands) · 2016Review
- First Report of the Occurrence of Trichinella-Specific Antibodies in Domestic Pigs in Central and Eastern Uganda.PloS one · 2016Article
- Early serodiagnosis of trichinellosis by ELISA using excretory-secretory antigens of Trichinella spiralis adult worms.Parasites & vectors · 2015Article
- Immunoproteomic profile of Trichinella spiralis adult worm proteins recognized by early infection sera.Parasites & vectors · 2015Article
- Assessing laboratory performance in Trichinella ring trials.Parasitology research · 2014Article
- Indirect versus direct detection methods of Trichinella spp. infection in wild boar (Sus scrofa).Parasites & vectors · 2014Article
- Subgroup J avian leukosis virus infection inhibits autophagy in DF-1 cells.Virology journal · 2013Article
- Trichinella sp. in red foxes (Vulpes vulpes) from Catalonia, NE Spain.Parasitology research · 2011Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trichinellosis is a zoonotic disease in humans caused by Trichinella spp. According to international regulations and guidelines, serological surveillance can be used to demonstrate the absence of Trichinella spp. in a defined domestic pig population. Most enzyme-linked immunosorbent assay (ELISA) tests presently available do not yield 100% specificity, and therefore, a complementary test is needed to confirm the diagnosis of any initial ELISA seropositivity. The goal of the present study was to evaluate the sensitivity and specificity of a Western Blot assay based on somatic Trichinella spiralis muscle stage (L1) antigen using Bayesian modeling techniques. A total of 295 meat juice and serum samples from pigs negative for Trichinella larvae by artificial digestion, including 74 potentially cross-reactive sera of pigs with other nematode infections, and 93 meat juice samples from pigs infected with Trichinella larvae were included in the study. The diagnostic sensitivity and specificity of the Western Blot were ranged from 95.8% to 96.0% and from 99.5% to 99.6%, respectively. A sensitivity analysis showed that the model outcomes were hardly influenced by changes in the prior distributions, providing a high confidence in the outcomes of the models. This validation study demonstrated that the Western Blot is a suitable method to confirm samples that reacted positively in an initial ELISA.
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