ArticleBritish journal of pharmacology1991
Characterization of diadenosine tetraphosphate (Ap4A) binding sites in cultured chromaffin cells: evidence for a P2y site.
Article in British journal of pharmacology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 63 citations in OpenAlex.
- Purinergic signalling in endocrine organs.Purinergic signalling · 2014Review
- Ca2+ signalling in brain synaptosomes activated by dinucleotides.The Journal of membrane biology · 2003Review
- Stimulatory effect of exogenous diadenosine tetraphosphate on insulin and glucagon secretion in the perfused rat pancreas.British journal of pharmacology · 1999Article
- Diadenosine polyphosphates evoke Ca2+ transients in guinea-pig brain via receptors distinct from those for ATP.The Journal of physiology · 1997Article
- Diadenosine polyphosphates induce intracellular Ca2+ mobilization in human neutrophils via a pertussis toxin sensitive G-protein.Immunology · 1997Article
- Selectivity and activity of adenine dinucleotides at recombinant P2X2 and P2Y1 purinoceptors.British journal of pharmacology · 1996Article
- Characterization of the binding of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to rat liver cell membranes.The Biochemical journal · 1996Article
- Cardiac ATP-sensitive K+ channel: a target for diadenosine 5',5''-P1,P5-pentaphosphate.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
- Effects of diadenosine polyphosphates, ATP and angiotensin II on cytosolic Ca2+ activity and contraction of rat mesangial cells.Pflugers Archiv : European journal of physiology · 1995Article
- Cytosolic free Ca2+ oscillations induced by diadenosine 5',5"'-P1,P3-triphosphate and diadenosine 5',5"'-P1,P4-tetraphosphate in single rat hepatocytes are indistinguishable from those induced by ADP and ATP respectively.The Biochemical journal · 1995Article
- A novel receptor for diadenosine polyphosphates coupled to calcium increase in rat midbrain synaptosomes.British journal of pharmacology · 1995Article
- Comparative studies on the affinities of ATP derivatives for P2x-purinoceptors in rat urinary bladder.British journal of pharmacology · 1994Article
- Nomenclature and classification of purinoceptors.Pharmacological reviews · 1994Review
- ATP modulation of calcium channels in chromaffin cells.The Journal of physiology · 1993Article
- Ap4A and ADP-beta-S binding to P2 purinoceptors present on rat brain synaptic terminals.British journal of pharmacology · 1993Article
- Ca(2+)-stores mobilization by diadenosine tetraphosphate, Ap4A, through a putative P2Y purinoceptor in adrenal chromaffin cells.British journal of pharmacology · 1992Article
- Effects of phorbol esters and secretagogues on nitrobenzylthioinosine binding to nucleoside transporters and nucleoside uptake in cultured chromaffin cells.The Biochemical journal · 1991Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Diadenosine tetraphosphate (Ap4A) a dinucleotide, which is stored in secretory granules, presents two types of high affinity binding sites in chromaffin cells. A Kd value of 8 +/- 0.65 x 10(-11) M and Bmax value of 5420 +/- 450 sites per cell were obtained for the high affinity binding site. A Kd value of 5.6 +/- 0.53 x 10(-9) M and a Bmax value close to 70,000 sites per cell were obtained for the second binding site with high affinity. 2. The diadenosine polyphosphates, Ap3A, Ap4A, Ap5A and Ap6A, displaced [3H]-Ap4A from the two binding sites, the Ki values being 1.0 nM, 0.013 nM, 0.013 nM and 0.013 nM for the very high affinity binding site and 0.5 microM, 0.13 microM, 0.062 microM and 0.75 microM for the second binding site. 3. The ATP analogues displaced [3H]-Ap4A with the potency order of the P2y receptors, adenosine 5'-O-(2 thiodiphosphate) (ADP-beta-S) greater than 5'-adenylyl imidodiphosphate (AMP-PNP) greater than alpha, beta-methylene ATP (alpha, beta-MeATP), in both binding sites. The Ki values were respectively 0.075 nM, 0.2 nM and 0.75 nM for the very high affinity binding site and 0.125 microM, 0.5 microM and 0.9 microM for the second binding site.
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