ArticlePlant physiology2009
Cell culture-induced gradual and frequent epigenetic reprogramming of invertedly repeated tobacco transgene epialleles.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Genetic and Global Epigenetic Modification, Which Determines the Phenotype of Transgenic Rice?International journal of molecular sciences · 2020Article
- Article
- 5-Azacytidine mediated reactivation of silenced transgenes in potato (Solanum tuberosum) at the whole plant level.Plant cell reports · 2017Article
- The origin of exon 3 skipping of paternal GLOBOSA pre-mRNA in some Nicotiana tabacum lines correlates with a point mutation of the very last nucleotide of the exon.Molecular genetics and genomics : MGG · 2016Article
- Leaf patterning of Clivia miniata var. variegata is associated with differential DNA methylation.Plant cell reports · 2016Article
- Article
- Effect of orientation of transcription of a gene in an inverted transferred DNA repeat on transcriptional gene silencing in rice transgenics-a case study.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
- Chromatin resetting mechanisms preventing transgenerational inheritance of epigenetic states.Frontiers in plant science · 2015Review
- The 2HA line of Medicago truncatula has characteristics of an epigenetic mutant that is weakly ethylene insensitive.BMC plant biology · 2014Article
- Progressive 35S promoter methylation increases rapidly during vegetative development in transgenic Nicotiana attenuata plants.BMC plant biology · 2013Article
- Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture.Epigenetics · 2013Article
- Tissue culture-induced genetic and epigenetic alterations in rice pure-lines, F1 hybrids and polyploids.BMC plant biology · 2013Article
- Epigenetic regulation of adaptive responses of forest tree species to the environment.Ecology and evolution · 2013Article
- HvCKX2 gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes.BMC plant biology · 2012Article
- An evolutionary view of plant tissue culture: somaclonal variation and selection.Plant cell reports · 2012Review
- Direct and indirect organogenesis of Clivia miniata and assessment of DNA methylation changes in various regenerated plantlets.Plant cell reports · 2012Article
- Coincident sequence-specific RNA degradation of linked transgenes in the plant genome.Plant molecular biology · 2012Article
- Article
- Genetic rearrangements can modify chromatin features at epialleles.PLoS genetics · 2011Article
- Paramutation of tobacco transgenes by small RNA-mediated transcriptional gene silencing.Epigenetics · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a two-component transgene system involving two epiallelic variants of the invertedly repeated transgenes in locus 1 (Lo1) and a homologous single-copy transgene locus 2 (Lo2), we have studied the stability of the methylation patterns and trans-silencing interactions in cell culture and regenerated tobacco (Nicotiana tabacum) plants. The posttranscriptionally silenced (PTGS) epiallele of the Lo1 trans-silences and trans-methylates the target Lo2 in a hybrid (Lo1/Lo2 line), while its transcriptionally silenced variant (Lo1E) does not. This pattern was stable over several generations in plants. However, in early Lo1E/Lo2 callus, decreased transgene expression and partial loss of Lo1E promoter methylation compared with leaf tissue in the parental plant were observed. Analysis of small RNA species and coding region methylation suggested that the transgenes were silenced by a PTGS mechanism. The Lo1/Lo2 line remained silenced, but the nonmethylated Lo1 promoter acquired partial methylation in later callus stages. These data indicate that a cell culture process has brought both epialleles to a similar epigenetic ground. Bisulfite sequencing of the 35S promoter within the Lo1 silencer revealed molecules with no, intermediate, and high levels of methylation, demonstrating, to our knowledge for the first time, cell-to-cell methylation diversity of callus. Regenerated plants showed high interindividual but low intraindividual epigenetic variability, indicating that the callus-induced epiallelic variants were transmitted to plants and became fixed. We propose that epigenetic changes associated with dedifferentiation might influence regulatory pathways mediated by trans-PTGS processes.
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