ArticlePlant physiology2009
The proteome of seed development in the model legume Lotus japonicus.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 88 citations in OpenAlex.
- Integrated physiological and transcriptomic measurements reveal changes in the accumulation and partitioning of storage reserves after prevention of pollination in maize.BMC plant biology · 2026Article
- Review
- Evaluation of the Susceptibility of Lotus Seeds (Toxins · 2024Article
- Spatio-temporal transcriptome and storage compound profiles of developing faba bean (Frontiers in plant science · 2024Article
- Underutilized legumes: nutrient status and advanced breeding approaches for qualitative and quantitative enhancement.Frontiers in nutrition · 2023Review
- Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach.Scientific reports · 2021Article
- Vegetative desiccation tolerance in the resurrection plant Xerophyta humilis has not evolved through reactivation of the seed canonical LAFL regulatory network.The Plant journal : for cell and molecular biology · 2020Article
- Carbohydrate reserves and seed development: an overview.Plant reproduction · 2018Review
- Towards Better Understanding of Pea Seed Dormancy Using Laser Desorption/Ionization Mass Spectrometry.International journal of molecular sciences · 2017Article
- Oil body biogenesis and biotechnology in legume seeds.Plant cell reports · 2017Review
- Regulation of Carbon Partitioning in the Seed of the Model LegumeFrontiers in plant science · 2017Article
- A Novel Phenolic Compound, Chloroxynil, Improves Agrobacterium-Mediated Transient Transformation in Lotus japonicus.PloS one · 2015Article
- Proteomic analysis of oil body membrane proteins accompanying the onset of desiccation phase during sunflower seed development.Plant signaling & behavior · 2015Article
- Proteomic analysis of embryogenesis and the acquisition of seed dormancy in Norway maple (Acer platanoides L.).International journal of molecular sciences · 2014Article
- Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis.Nature protocols · 2014Article
- Article
- Article
- Advances in omics and bioinformatics tools for systems analyses of plant functions.Plant & cell physiology · 2011Review
- Article
- Recent Progress in Development of Tnt1 Functional Genomics Platform for Medicago truncatula and Lotus japonicus in Bulgaria.Current genomics · 2011Article
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean (Glycine max) and pea (Pisum sativum), Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic three developmental phases of legume seeds and the presence of embryo, endosperm, and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined, and this indicates that the composition of mature Lotus seed is more similar to soybean than to pea. In a first attempt to determine the seed proteome, both a two-dimensional polyacrylamide gel electrophoresis approach and a gel-based liquid chromatography-mass spectrometry approach were used. Globulins were analyzed by two-dimensional polyacrylamide gel electrophoresis, and five legumins, LLP1 to LLP5, and two convicilins, LCP1 and LCP2, were identified by matrix-assisted laser desorption ionization quadrupole/time-of-flight mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a gel-based liquid chromatography-mass spectrometry approach was used, and 665 and 181 unique proteins corresponding to gene accession numbers were identified for the two phases, respectively. All of the proteome data, including the experimental data and mass spectrometry spectra peaks, were collected in a database that is available to the scientific community via a Web interface (http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi). This database establishes the basis for relating physiology, biochemistry, and regulation of seed development in Lotus. Together with a new Web interface (http://bioinfoserver.rsbs.anu.edu.au/utils/PathExpress4legumes/) collecting all protein identifications for Lotus, Medicago, and soybean seed proteomes, this database is a valuable resource for comparative seed proteomics and pathway analysis within and beyond the legume family.
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