ArticlePlant physiology2009
Postillumination isoprene emission: in vivo measurements of dimethylallyldiphosphate pool size and isoprene synthase kinetics in aspen leaves.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 95 citations in OpenAlex.
- Mechanisms of Isoprene Decoupling in Poplar: Precursor Dynamics and VOC Fluxes Under Acute Thermal Exposure and Elevated COPlants (Basel, Switzerland) · 2026Article
- Biosynthetic Machinery to Abiotic Stress-Driven Emission: Decoding Multilayer Regulation of Volatile Terpenoids in Plants.Antioxidants (Basel, Switzerland) · 2025Review
- Limitations of Plant Stress Tolerance upon Heat and COPlants (Basel, Switzerland) · 2024Article
- Hydroxymethylbutenyl diphosphate accumulation reveals MEP pathway regulation for high COProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Exposure to different light intensities affects emission of volatiles and accumulations of both pigments and phenolics in Azolla filiculoides.Physiologia plantarum · 2022Article
- Responses of isoprene emission and photochemical efficiency to severe drought combined with prolonged hot weather in hybrid Populus.Journal of experimental botany · 2020Article
- Isoprene: New insights into the control of emission and mediation of stress tolerance by gene expression.Plant, cell & environment · 2019Review
- Isoprene Acts as a Signaling Molecule in Gene Networks Important for Stress Responses and Plant Growth.Plant physiology · 2019Article
- Why Algae Release Volatile Organic Compounds-The Emission and Roles.Frontiers in microbiology · 2019Review
- Alternative Carbon Sources for Isoprene Emission.Trends in plant science · 2018Review
- Temporal regulation of terpene synthase gene expression inEnvironmental and experimental botany · 2018Article
- Evidence That Isoprene Emission Is Not Limited by Cytosolic Metabolites. Exogenous Malate Does Not Invert the Reverse Sensitivity of Isoprene Emission to High [COPlant physiology · 2018Article
- Ozone-triggered surface uptake and stress volatile emissions in Nicotiana tabacum 'Wisconsin'.Journal of experimental botany · 2018Article
- Differential regulation of volatile emission fromEnvironmental and experimental botany · 2018Article
- Ozone-induced foliar damage and release of stress volatiles is highly dependent on stomatal openness and priming by low-level ozone exposure in Phaseolus vulgaris.Plant, cell & environment · 2017Article
- Article
- Spectacular Oscillations in Plant Isoprene Emission under Transient Conditions Explain the Enigmatic CO2 Response.Plant physiology · 2016Article
- Herbivory by an Outbreaking Moth Increases Emissions of Biogenic Volatiles and Leads to Enhanced Secondary Organic Aerosol Formation Capacity.Environmental science & technology · 2016Article
- Controls of the quantum yield and saturation light of isoprene emission in different-aged aspen leaves.Plant, cell & environment · 2015Article
- Bisphosphonate inhibitors reveal a large elasticity of plastidic isoprenoid synthesis pathway in isoprene-emitting hybrid aspen.Plant physiology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The control of foliar isoprene emission is shared between the activity of isoprene synthase, the terminal enzyme catalyzing isoprene formation from dimethylallyldiphosphate (DMADP), and the pool size of DMADP. Due to limited in vivo information of isoprene synthase kinetic characteristics and DMADP pool sizes, the relative importance of these controls is under debate. In this study, the phenomenon of postillumination isoprene release was employed to develop an in vivo method for estimation of the DMADP pool size and to determine isoprene synthase kinetic characteristics in hybrid aspen (Populus tremula x Populus tremuloides) leaves. The method is based on observations that after switching off the light, isoprene emission continues for 250 to 300 s and that the integral of the postillumination isoprene emission is strongly correlated with the isoprene emission rate before leaf darkening, thus quantitatively estimating the DMADP pool size associated with leaf isoprene emission. In vitro estimates demonstrated that overall leaf DMADP pool was very large, almost an order of magnitude larger than the in vivo pool. Yet, the difference between total DMADP pools in light and in darkness (light-dependent DMADP pool) was tightly correlated with the in vivo estimates of the DMADP pool size that is responsible for isoprene emission. Variation in in vivo DMADP pool size was obtained by varying light intensity and atmospheric CO(2) and O(2) concentrations. From these experiments, the in vivo kinetic constants of isoprene synthase were determined. In vivo isoprene synthase kinetic characteristics suggested that isoprene synthase mainly operates under substrate limitation and that short-term light, CO(2), and O(2) dependencies of isoprene emission result from variation in DMADP pool size rather than from modifications in isoprene synthase activity.
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