ArticleJournal of clinical microbiology2009
Unreliability of results of PCR detection of Helicobacter pylori in clinical or environmental samples.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- Differential effect of Helicobacter pylori eradication on time-trends in brady/hypokinesia and rigidity in idiopathic parkinsonism.Helicobacter · 2010Trial
- Distribution of Helicobacter pylori in the Oral Cavity of Patients With Tooth Extractions at Various Locations and Ages.International dental journal · 2026Article
- Peculiarities of Diagnostic Reliability-Nested PCR Versus SAT in the Identification ofMicroorganisms · 2025Article
- The link betweenAnnals of hepato-biliary-pancreatic surgery · 2023Review
- Association of Chronic Periodontitis with Helicobacter pylori Infection in Stomach or Mouth: A Systematic Review and Meta-Analysis.European journal of dentistry · 2023Article
- Analysis of Genetic Relatedness between Gastric and OralInternational journal of molecular sciences · 2023Article
- Helicobacter pylori Infection, Its Laboratory Diagnosis, and Antimicrobial Resistance: a Perspective of Clinical Relevance.Clinical microbiology reviews · 2022Review
- The validity evaluation of different 16srRNA gene primers for helicobacter detection urgently requesting to design new specific primers.Scientific reports · 2022Article
- Tumor tissueDental research journal · 2022Article
- Comparison of invasive histological and molecular methods in the diagnosis ofF1000Research · 2022Article
- DNA diagnostics for reliable and universal identification ofWorld journal of gastroenterology · 2021Review
- Demographic, Chemical, andCanadian journal of gastroenterology & hepatology · 2021Article
- Current Prevalence of OralPathogens (Basel, Switzerland) · 2020Article
- Analysis of by high-throughput sequencing: Helicobacter pylori infection and salivary microbiome.BMC oral health · 2020Article
- Association between Helicobacter pylori infection and dental pulp reservoirs in Japanese adults.BMC oral health · 2019Article
- Potential transmission sources ofJournal of environmental health science & engineering · 2019Article
- Application of PCR and Microscopy to DetectThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2019Article
- Molecular and clinical analyses of Helicobacter pylori colonization in inflamed dental pulp.BMC oral health · 2018Article
- An In Silico Approach for Identification of the Pathogenic Species, Helicobacter pylori and Its Relatives.Indian journal of microbiology · 2016Article
- Establishment of a nested-ASP-PCR method to determine the clarithromycin resistance of Helicobacter pylori.World journal of gastroenterology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
The aim of this study was to compare published Helicobacter pylori primer pairs for their ability to reliably detect H. pylori in gastric biopsy specimens and salivary samples. Detection limits of the 26 PCR primer pairs previously described for detection of H. pylori DNA in clinical samples were determined. Sensitivity and specificity were determined using primers with detection limits of <100 CFU/ml using 50 H. pylori-positive and -negative (by concordance by culture and histology) coded gastric biopsy specimens. These results were then confirmed with gastric biopsy specimens and saliva from patients with confirmed H. pylori status. Five of the twenty-six previously reported primer pairs (HP64-f/HP64-r, HP1/HP2, EHC-U/EHC-L, VAG-F/VAG-R, and ICT37/ICT38) had detection limits of <100 CFU/ml in the presence of gastric tissue. None had 100% specificity or sensitivity; all produced false-positive results. The HP64-f/HP64-r for ureA and HP1/HP2 for 16S rRNA individually had sensitivities and specificities of >90% with gastric biopsy specimens. No combinations of primer pairs improved the results. Using these five primer pairs, 54% of the positive saliva samples were determined to be false positive; both the HP64-f/HP64-r and the HP1/HP2 sets produced false positives with saliva. We conclude that clinicians should not rely on results using current PCR primers alone to decide the H. pylori status of an individual patient or as a basis for treatment decisions. The results of studies based on PCR identification of H. pylori in environmental samples should be viewed with caution. Possibly, specific primers sets can be identified based on the presence of multiple putative virulence factor genes.
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Registered trials
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