ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Matrix-dependent local retention of secretory vesicle cargo in cortical neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 71 citations in OpenAlex.
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- IGF-like growth factors that couple food sensing to developmental decisions employ different release mechanisms in a single pair ofbioRxiv : the preprint server for biology · 2026Article
- Neuronal Network Inactivity Potentiates Neuropeptide Release from Mouse Cortical Neurons.eNeuro · 2025Article
- Article
- Maximal Fusion Capacity and Efficient Replenishment of the Dense Core Vesicle Pool in Hippocampal Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Modulations of the neuronal trafficking of tissue-type plasminogen activator (tPA) influences glutamate release.Cell death & disease · 2023Article
- The SNARE protein SNAP-25 is required for normal exocytosis at auditory hair cell ribbon synapses.iScience · 2022Article
- Vti1a/b support distinct aspects of TGN and cis-/medial Golgi organization.Scientific reports · 2022Article
- Article
- Neuromodulator release in neurons requires two functionally redundant calcium sensors.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons.STAR protocols · 2021Article
- Functional Implications of Neurotransmitter Segregation.Frontiers in neural circuits · 2021Review
- The physiology of regulated BDNF release.Cell and tissue research · 2020Review
- Tetanus insensitive VAMP2 differentially restores synaptic and dense core vesicle fusion in tetanus neurotoxin treated neurons.Scientific reports · 2020Article
- Real-time imaging of multivesicular body-plasma membrane fusion to quantify exosome release from single cells.Nature protocols · 2020Article
- Article
- Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- Pool size estimations for dense-core vesicles in mammalian CNS neurons.The EMBO journal · 2018Article
- Secretory vesicle trafficking in awake and anaesthetized mice: differential speeds in axons versus synapses.The Journal of physiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Neurons secrete many diffusible signals from synaptic and other secretory vesicles. We characterized secretion of guidance cues, neuropeptides, neurotrophins, and proteases from single secretory vesicles using pHluorin-tagged cargo in cortical neurons. Stimulation triggered transient and persistent fusion events. Transient events represented full release followed by cargo diffusion or incomplete release followed by vesicle retrieval, as previously observed in neuroendocrine cells. Unexpectedly, we also observed that certain cargo, such as Semaphorin 3A (Sema3A), was delivered at the cell surface as stable deposits. Stable deposits and transient events were observed for single cargo and both were SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) and calcium dependent. The ratio between stable and transient events did not depend on cargo size, subcellular localization (synaptic vs extrasynaptic secretion), or the presence of the extracellular matrix. Instead, the ratio is cargo specific and depends on an interaction with the vesicle matrix through a basic domain in the cargo protein. Inhibition of this interaction through deletion of the basic domain in Sema3A abolished stable deposits and rendered all events transient. Strikingly, cargo favoring transient release was stably deposited after corelease with cargo favoring stable deposit. These data argue against cargo diffusion after exocytosis as a general principle. Instead, the vesicle matrix retains secreted signals, probably for focal signaling at the cell surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.