ArticleNucleic acids research2009
The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 133 papers.
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Who cites it
133 citing papers in PubMed, 217 citations in OpenAlex.
- Review
- New insights into nuclear import and nucleolar localization of yeast RNA exosome subunits.Molecular biology of the cell · 2025Article
- Article
- Functional characterization of human recessive DIS3 variants in premature ovarian insufficiency†.Biology of reproduction · 2025Article
- RPA transforms RNase H1 to a bidirectional exoribonuclease for processive RNA-DNA hybrid cleavage.Nature communications · 2024Article
- Article
- How hydrolytic exoribonucleases impact human disease: Two sides of the same story.FEBS open bio · 2023Review
- Review
- Trypanosoma brucei RRP44: a versatile enzyme for processing structured and non-structured RNA substrates.Nucleic acids research · 2023Article
- Dissecting Trypanosoma brucei RRP44 function in the maturation of segmented ribosomal RNA using a regulated genetic complementation system.Nucleic acids research · 2023Article
- Control of RNA degradation in cell fate decision.Frontiers in cell and developmental biology · 2023Review
- MPP6 stimulates both RRP6 and DIS3 to degrade a specified subset of MTR4-sensitive substrates in the human nucleus.Nucleic acids research · 2022Article
- DIS3 mutations in multiple myeloma impact the transcriptional signature and clinical outcome.Haematologica · 2022Article
- RNA metabolism and links to inflammatory regulation and disease.Cellular and molecular life sciences : CMLS · 2021Review
- RNA-regulatory exosome complex confers cellular survival to promote erythropoiesis.Nucleic acids research · 2021Article
- Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A Conserved NRDE-2/MTR-4 Complex Mediates Nuclear RNAi inGenetics · 2020Article
- Dis3L2 regulates cell proliferation and tissue growth through a conserved mechanism.PLoS genetics · 2020Article
- Molecular characteristics and functional differences of anti-PM/Scl autoantibodies and two other distinct and unique supramolecular structures known as "EXOSOMES".Autoimmunity reviews · 2020Review
- RNA Exosomes and Their Cofactors.Methods in molecular biology (Clifton, N.J.) · 2020Review
73 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Nuclear and cytoplasmic forms of the yeast exosome share 10 components, of which only Rrp44/Dis3 is believed to possess 3' exonuclease activity. We report that expression only of Rrp44 lacking 3'-exonuclease activity (Rrp44-exo) supports growth in S288c-related strains (BY4741). In BY4741, rrp44-exo was synthetic-lethal with loss of the cytoplasmic 5'-exonuclease Xrn1, indicating block of mRNA turnover, but not with loss of the nuclear 3'-exonuclease Rrp6. The RNA processing phenotype of rrp44-exo was milder than that seen on Rrp44 depletion, indicating that Rrp44-exo retains important functions. Recombinant Rrp44 was shown to possess manganese-dependent endonuclease activity in vitro that was abolished by four point mutations in the putative metal binding residues of its N-terminal PIN domain. Rrp44 lacking both exonuclease and endonuclease activity failed to support growth in strains depleted of endogenous Rrp44. Strains expressing Rrp44-exo and Rrp44-endo-exo exhibited different RNA processing patterns in vivo suggesting Rrp44-dependent endonucleolytic cleavages in the 5'-ETS and ITS2 regions of the pre-rRNA. Finally, the N-terminal PIN domain was shown to be necessary and sufficient for association with the core exosome, indicating its dual function as a nuclease and structural element.
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