ArticleNucleic acids research2009
Oxidation of a single active site suffices for the functional inactivation of the dimeric Bacillus subtilis OhrR repressor in vitro.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Review
- Positioning of extracellular loop 1 affects pore gating of the cystic fibrosis transmembrane conductance regulator.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Structure of the MarR family protein Rv0880 from Mycobacterium tuberculosis.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Protein topology determines cysteine oxidation fate: the case of sulfenyl amide formation among protein families.PLoS computational biology · 2015Article
- Thiol-based redox switches.Biochimica et biophysica acta · 2014Review
- Redox regulation in Bacillus subtilis: The bacilliredoxins BrxA(YphP) and BrxB(YqiW) function in de-bacillithiolation of S-bacillithiolated OhrR and MetE.Antioxidants & redox signaling · 2014Article
- Thiol redox biochemistry: insights from computer simulations.Biophysical reviews · 2014Review
- Peroxide-sensing transcriptional regulators in bacteria.Journal of bacteriology · 2012Review
- Characterization of the organic hydroperoxide resistance system of Brucella abortus 2308.Journal of bacteriology · 2012Article
- Structure of the regulatory domain of the LysR family regulator NMB2055 (MetR-like protein) from Neisseria meningitidis.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Resistance to organic hydroperoxides requires ohr and ohrR genes in Sinorhizobium meliloti.BMC microbiology · 2011Article
- Thiol-based redox switches and gene regulation.Antioxidants & redox signaling · 2011Review
- DNA-mediated charge transport in redox sensing and signaling.Journal of the American Chemical Society · 2010Article
- Unnatural amino acid substitution as a probe of the allosteric coupling pathway in a mycobacterial Cu(I) sensor.Journal of the American Chemical Society · 2009Article
- Energetics of allosteric negative coupling in the zinc sensor S. aureus CzrA.Journal of the American Chemical Society · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis OhrR is a dimeric repressor that senses organic peroxides and regulates the expression of the OhrA peroxiredoxin. Derepression results from oxidation of an active site cysteine which ultimately results in formation of a mixed disulfide with a low molecular weight thiol, a cyclic sulfenamide, or overoxidation to the sulfinic or sulfonic acids. We expressed a single-chain OhrR (scOhrR) in which the two monomers were connected by a short amino-acid linker. scOhrR variants containing only one active site cysteine were fully functional as repressors and still responded, albeit with reduced efficacy, to organic peroxides in vivo. Biochemical analyses indicate that oxidation at a single active site is sufficient for derepression regardless of the fate of the active site cysteine. scOhrR with only one active site cysteine in the amino-terminal domain is inactivated at rates comparable to wild-type whereas when the active site is in the carboxyl-terminal domain the protein is inactivated much more slowly. The incomplete derepression noted for single active site variants of scOhrR in vivo is consistent with the hypothesis that protein reduction regenerates active repressor and that, in the cell, oxidation of the second active site may also contribute to derepression.
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