ArticleThe Journal of biological chemistry2009
Homology with vesicle fusion mediator syntaxin-1a predicts determinants of epimorphin/syntaxin-2 function in mammary epithelial morphogenesis.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Exploring of bladder cancer immune-related genes and potential therapeutic targets based on transcriptomic data and Mendelian randomization analysis.Frontiers in immunology · 2025Article
- Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.Nature communications · 2022Article
- STX2 Promotes Trophoblast Growth, Migration, and Invasion Through Activation of the PI3K-AKT Pathway in Preeclampsia.Frontiers in cell and developmental biology · 2021Article
- Syntaxin 2 promotes colorectal cancer growth by increasing the secretion of exosomes.Journal of Cancer · 2021Article
- STX2 promotes colorectal cancer metastasis through a positive feedback loop that activates the NF-κB pathway.Cell death & disease · 2018Article
- Membrane translocation of t-SNARE protein syntaxin-4 abrogates ground-state pluripotency in mouse embryonic stem cells.Scientific reports · 2017Article
- Article
- Extracellularly Extruded Syntaxin-4 Is a Potent Cornification Regulator of Epidermal Keratinocytes.Molecular medicine (Cambridge, Mass.) · 2015Article
- Regulation of epithelial-mesenchymal transition in breast cancer cells by cell contact and adhesion.Cancer informatics · 2015Article
- Epimorphin expression in a rat model of pulmonary hypoplasia associated with congenital diaphragmatic hernia.Pediatric surgery international · 2014Article
- ROS-induced epithelial-mesenchymal transition in mammary epithelial cells is mediated by NF-kB-dependent activation of Snail.Oncotarget · 2014Article
- Tumor cell-produced matrix metalloproteinase 9 (MMP-9) drives malignant progression and metastasis of basal-like triple negative breast cancer.Oncotarget · 2014Article
- Epimorphin deletion inhibits polyposis in the Apcmin/+ mouse model of colon carcinogenesis via decreased myofibroblast HGF secretion.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- Epimorphin is a novel regulator of the progesterone receptor isoform-a.Cancer research · 2013Article
- PI3K regulates branch initiation and extension of cultured mammary epithelia via Akt and Rac1 respectively.Developmental biology · 2013Article
- Article
- Growth of lung cancer cells in three-dimensional microenvironments reveals key features of tumor malignancy.Integrative biology : quantitative biosciences from nano to macro · 2012Article
- Epithelial-mesenchymal transition: general principles and pathological relevance with special emphasis on the role of matrix metalloproteinases.Cold Spring Harbor perspectives in biology · 2012Review
- Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis.The EMBO journal · 2011Article
- Matrix metalloproteinase-induced epithelial-mesenchymal transition in breast cancer.Journal of mammary gland biology and neoplasia · 2010Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
We have shown that branching morphogenesis of mammary ductal structures requires the action of the morphogen epimorphin/syntaxin-2. Epimorphin, originally identified as an extracellular molecule, is identical to syntaxin-2, an intracellular molecule that is a member of the extensively investigated syntaxin family of proteins that mediate vesicle trafficking. We show here that, although epimorphin/syntaxin-2 is highly homologous to syntaxin-1a, only epimorphin/syntaxin-2 can stimulate mammary branching morphogenesis. We construct a homology model of epimorphin/syntaxin-2 based on the published structure of syntaxin-1a, and we use this model to identify the structural motif responsible for the morphogenic activity. We identify four residues located within the cleft between helices B and C that differ between syntaxin-1a and epimorphin/syntaxin-2; through site-directed mutagenesis of these four amino acids, we confer the properties of epimorphin for cell adhesion, gene activation, and branching morphogenesis onto the inactive syntaxin-1a template. These results provide a dramatic demonstration of the use of structural information about one molecule to define a functional motif of a second molecule that is related at the sequence level but highly divergent functionally.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.