ArticleJournal of experimental botany2009
A green fluorescent protein fused to rice prolamin forms protein body-like structures in transgenic rice.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 47 citations in OpenAlex.
- Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria.Transgenic research · 2020Article
- Enzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier.Scientific reports · 2019Article
- Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm.Plant & cell physiology · 2019Article
- Proteomic Data Integration Highlights Central Actors Involved in Einkorn (Frontiers in plant science · 2019Article
- Specific region affects the difference in accumulation levels between apple food allergen Mal d 1 and birch pollen allergen Bet v 1 which are expressed in vegetative tissues of transgenic rice.Plant molecular biology · 2018Article
- Maize 16-kD γ-zein forms very unusual disulfide-bonded polymers in the endoplasmic reticulum: implications for prolamin evolution.Journal of experimental botany · 2018Article
- Expression of the Nitrate Transporter GeneThe Plant cell · 2018Article
- Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain.International journal of molecular sciences · 2017Article
- Accumulation of foreign polypeptides to rice seed protein body type I using prolamin portion sequences.Plant cell reports · 2017Article
- Control of foreign polypeptide localization in specific layers of protein body type I in rice seed.Plant cell reports · 2016Article
- Rice endosperm produces an underglycosylated and potent form of the HIV-neutralizing monoclonal antibody 2G12.Plant biotechnology journal · 2016Article
- Where do Protein Bodies of Cereal Seeds Come From?Frontiers in plant science · 2016Review
- Enhanced Sucrose Loading Improves Rice Yield by Increasing Grain Size.Plant physiology · 2015Article
- Article
- Protein accumulation in aleurone cells, sub-aleurone cells and the center starch endosperm of cereals.Plant cell reports · 2014Review
- The trafficking pathway of a wheat storage protein in transgenic rice endosperm.Annals of botany · 2014Article
- Variation in the Subcellular Localization and Protein Folding Activity among Arabidopsis thaliana Homologs of Protein Disulfide Isomerase.Biomolecules · 2013Article
- Protein bodies in nature and biotechnology.Molecular biotechnology · 2013Article
- Accumulation of rice prolamin-GFP fusion proteins induces ER-derived protein bodies in transgenic rice calli.Plant cell reports · 2013Article
- Comparative study of wheat low-molecular-weight glutenin and α-gliadin trafficking in tobacco cells.Plant cell reports · 2013Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prolamins, a group of rice (Oryza sativa) seed storage proteins, are synthesized on the rough endoplasmic reticulum (ER) and deposited in ER-derived type I protein bodies (PB-Is) in rice endosperm cells. The accumulation mechanism of prolamins, which do not possess the well-known ER retention signal, remains unclear. In order to elucidate whether the accumulation of prolamin in the ER requires seed-specific factors, the subcellular localization of the constitutively expressed green fluorescent protein fused to prolamin (prolamin-GFP) was examined in seeds, leaves, and roots of transgenic rice plants. The prolamin-GFP fusion proteins accumulated not only in the seeds but also in the leaves and roots. Microscopic observation of GFP fluorescence and immunocytochemical analysis revealed that prolamin-GFP fusion proteins specifically accumulated in PB-Is in the endosperm, whereas they were deposited in the electron-dense structures in the leaves and roots. The ER chaperone BiP was detected in the structures in the leaves and roots. The results show that the aggregation of prolamin-GFP fusion proteins does not depend on the tissues, suggesting that the prolamin-GFP fusion proteins accumulate in the ER by forming into aggregates. The findings bear out the importance of the assembly of prolamin molecules and the interaction of prolamin with BiP in the formation of ER-derived PBs.
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