ArticleJournal of experimental botany2009
Tobacco Arp3 is localized to actin-nucleating sites in vivo.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Tobacco two-pore calcium channel 1a is localised at the tonoplast, but acts on events at the plasma membrane.Protoplasma · 2026Article
- ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants.Nature plants · 2023Article
- Kinetin induces microtubular breakdown, cell cycle arrest and programmed cell death in tobacco BY-2 cells.Protoplasma · 2023Article
- Cell type matters: competence for alkaloid metabolism differs in two seed-derived cell strains of Catharanthus roseus.Protoplasma · 2023Article
- Cell wall and cytoskeletal contributions in single cell biomechanics ofQuantitative plant biology · 2022Article
- Dissecting the membrane-microtubule sensor in grapevine defence.Horticulture research · 2021Article
- Hunting modulators of plant defence: the grapevine trunk disease fungus Eutypa lata secretes an amplifier for plant basal immunity.Journal of experimental botany · 2020Article
- The tomato Arp2/3 complex is required for resistance to the powdery mildew fungus Oidium neolycopersici.Plant, cell & environment · 2019Article
- The WASP-Arp2/3 complex signal cascade is involved in actin-dependent sperm nuclei migration during double fertilization in tobacco and maize.Scientific reports · 2017Article
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- Heterodimeric capping protein from Arabidopsis is a membrane-associated, actin-binding protein.Plant physiology · 2014Article
- Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf Trichomes.The Plant cell · 2014Article
- ARP2/3 localization in Arabidopsis leaf pavement cells: a diversity of intracellular pools and cytoskeletal interactions.Frontiers in plant science · 2013Article
- The phytoalexin resveratrol regulates the initiation of hypersensitive cell death in Vitis cell.PloS one · 2011Article
- A kinesin with calponin-homology domain is involved in premitotic nuclear migration.Journal of experimental botany · 2010Article
- Probing the actin-auxin oscillator.Plant signaling & behavior · 2010Review
- Light can rescue auxin-dependent synchrony of cell division in a tobacco cell line.Journal of experimental botany · 2010Article
- The association of the Arabidopsis actin-related protein2/3 complex with cell membranes is linked to its assembly status but not its activation.Plant physiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polarity of actin is a central determinant of intracellular transport in plant cells. To visualize actin polarity in living plant cells, the tobacco homologue of the actin-related protein 3 (ARP3) was cloned and a fusion with the red fluorescent protein (RFP) was generated. Upon transient expression of these fusions in the tobacco cell line BY-2 (Nicotiana tabacum L. cv. Bright Yellow 2), punctate structures were observed near the nuclear envelope and in the cortical plasma. These dots could be shown to decorate actin filaments by expressing RFP-ARP3 in a marker line, where actin was tagged by GFP (green fluorescent protein)-FABD (fimbrin actin-binding domain 2). When actin filaments were disrupted by latrunculin B or by prolonged cold treatment, and subsequently allowed to recover, the actin filaments reformed from the RFP-ARP3 structures, that therefore represented actin nucleation sites. The intracellular distribution of these sites was followed during the formation of pluricellular files, and it was observed that the density of RFP-ARP3 increased in the apex of the polarized, terminal cells of a file, whereas it was equally distributed in the central cells of a file. These findings are interpreted in terms of position-dependent differences of actin organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.