ArticleBMC plant biology2009
A multiple-method approach reveals a declining amount of chloroplast DNA during development in Arabidopsis.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 51 citations in OpenAlex.
- The Potential Role of Plastome Copy Number as a Quality Biomarker for Plant Products using Real-time Quantitative Polymerase Chain Reaction.Current genomics · 2022Article
- Detecting de novo mitochondrial mutations in angiosperms with highly divergent evolutionary rates.Genetics · 2021Article
- Changes in plastid biogenesis leading to the formation of albino regenerants in barley microspore culture.BMC plant biology · 2021Article
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Plant Organelle Genome Replication.Plants (Basel, Switzerland) · 2019Review
- A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity information.BMC microbiology · 2019Article
- The Mitochondrial Endonuclease M20 Participates in the Down-Regulation of Mitochondrial DNA in Pollen Cells.Plant physiology · 2018Article
- Optimized Method of Extracting Rice Chloroplast DNA for High-Quality Plastome Resequencing andFrontiers in plant science · 2018Article
- Real-Time PCR Quantification of Chloroplast DNA Supports DNA Barcoding of Plant Species.Molecular biotechnology · 2016Article
- High-throughput multiplex cpDNA resequencing clarifies the genetic diversity and genetic relationships among Brassica napus, Brassica rapa and Brassica oleracea.Plant biotechnology journal · 2016Article
- Organellar genome copy number variation and integrity during moderate maturation of roots and leaves of maize seedlings.Current genetics · 2015Article
- DNA maintenance in plastids and mitochondria of plants.Frontiers in plant science · 2015Review
- Changes in DNA damage, molecular integrity, and copy number for plastid DNA and mitochondrial DNA during maize development.Journal of experimental botany · 2014Article
- On the fate of plastid DNA molecules during leaf development: response to the Golczyk et al. Commentary.The Plant cell · 2014Article
- Chloroplast DNA in mature and senescing leaves: a reappraisal.The Plant cell · 2014Article
- Article
- Effects of reduced chloroplast gene copy number on chloroplast gene expression in maize.Plant physiology · 2012Article
- Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing.BMC biotechnology · 2011Article
- Divergent roles for the two PolI-like organelle DNA polymerases of Arabidopsis.Plant physiology · 2011Article
- A conserved, Mg²+-dependent exonuclease degrades organelle DNA during Arabidopsis pollen development.The Plant cell · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundA decline in chloroplast DNA (cpDNA) during leaf maturity has been reported previously for eight plant species, including Arabidopsis thaliana. Recent studies, however, concluded that the amount of cpDNA during leaf development in Arabidopsis remained constant.
resultsTo evaluate alternative hypotheses for these two contradictory observations, we examined cpDNA in Arabidopsis shoot tissues at different times during development using several methods: staining leaf sections as well as individual isolated chloroplasts with 4',6-diamidino-2-phenylindole (DAPI), real-time quantitative PCR with DNA prepared from total tissue as well as from isolated chloroplasts, fluorescence microscopy of ethidium-stained DNA molecules prepared in gel from isolated plastids, and blot-hybridization of restriction-digested total tissue DNA. We observed a developmental decline of about two- to three-fold in mean DNA per chloroplast and two- to five-fold in the fraction of cellular DNA represented by chloroplast DNA.
conclusionSince the two- to five-fold reduction in cpDNA content could not be attributed to an artifact of chloroplast isolation, we conclude that DNA within Arabidopsis chloroplasts is degraded in vivo as leaves mature.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.