ArticlePLoS biology2009
An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexes.
Article in PLoS biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- Composite SMG5-SMG6 PIN domain formation is essential for NMD.Nature communications · 2026Article
- Unraveling the causal genes and transcriptomic determinants of human telomere length.Nature communications · 2023Article
- Nuclear mRNA Quality Control and Cytoplasmic NMD Are Linked by the Guard Proteins Gbp2 and Hrb1.International journal of molecular sciences · 2021Review
- Writing a wrong: Coupled RNA polymerase II transcription and RNA quality control.Wiley interdisciplinary reviews. RNA · 2019Review
- IMiQ: a novel protein quality control compartment protecting mitochondrial functional integrity.Molecular biology of the cell · 2018Article
- Novel functions for chromatin dynamics in mRNA biogenesis beyond transcription.Nucleus (Austin, Tex.) · 2017Review
- Structural conservation of the PIN domain active site across all domains of life.Protein science : a publication of the Protein Society · 2017Review
- Comprehensive classification of the PIN domain-like superfamily.Nucleic acids research · 2017Article
- The PIN domain endonuclease Utp24 cleaves pre-ribosomal RNA at two coupled sites in yeast and humans.Nucleic acids research · 2016Article
- Posttranslational marks control architectural and functional plasticity of the nuclear pore complex basket.The Journal of cell biology · 2016Article
- Nuclear pore components affect distinct stages of intron-containing gene expression.Nucleic acids research · 2015Article
- Nuclear export of messenger RNA.Genes · 2015Review
- hUTP24 is essential for processing of the human rRNA precursor at site A1, but not at site A0.RNA biology · 2015Article
- Intron or no intron: a matter for nuclear pore complexes.Nucleus (Austin, Tex.) · 2015Article
- Rio1 mediates ATP-dependent final maturation of 40S ribosomal subunits.Nucleic acids research · 2014Article
- Review
- mRNA stability in the nucleus.Journal of Zhejiang University. Science. B · 2014Review
- The nuclear basket proteins Mlp1p and Mlp2p are part of a dynamic interactome including Esc1p and the proteasome.Molecular biology of the cell · 2013Article
- Yeast hEST1A/B (SMG5/6)-like proteins contribute to environment-sensing adaptive gene expression responses.G3 (Bethesda, Md.) · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear mRNA export is a crucial step in eukaryotic gene expression, which is in yeast coupled to cotranscriptional messenger ribonucleoprotein particle (mRNP) assembly and surveillance. Several surveillance systems that monitor nuclear mRNP biogenesis and export have been described, but the mechanism by which the improper mRNPs are recognized and eliminated remains poorly understood. Here we report that the conserved PIN domain protein Swt1 is an RNA endonuclease that participates in quality control of nuclear mRNPs and can associate with the nuclear pore complex (NPC). Swt1 showed endoribonuclease activity in vitro that was inhibited by a point mutation in the predicted catalytic site. Swt1 lacked clear sequence specificity but showed a strong preference for single-stranded regions. Genetic interactions were found between Swt1 and the THO/TREX and TREX-2 complexes, and with components of the perinuclear mRNP surveillance system, Mlp1, Nup60, and Esc1. Inhibition of the nuclease activity of Swt1 increased the levels and cytoplasmic leakage of unspliced aberrant pre-mRNA, and induced robust nuclear poly(A)(+) RNA accumulation in mlp1Delta and esc1Delta strains. Overexpression of Swt1 also caused strong nuclear poly(A)(+) RNA accumulation. Swt1 is normally distributed throughout the nucleus and cytoplasm but becomes concentrated at nuclear pore complexes (NPCs) in the nup133Delta mutant, which causes NPC clustering and defects in mRNP export. The data suggest that Swt1 endoribonuclease might be transiently recruited to NPCs to initiate the degradation of defective pre-mRNPs or mRNPs trapped at nuclear periphery in order to avoid their cytoplasmic export and translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.