ArticleThe Korean journal of parasitology2008
Contact-independent cell death of human microglial cells due to pathogenic Naegleria fowleri trophozoites.
Article in The Korean journal of parasitology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- In Situ Cytokine Expression, Nitric Oxide and Reactive Oxygen Species Production During Primary Amoebic Meningoencephalitis Development.International journal of molecular sciences · 2026Article
- Advances in the study of extracellular vesicles of Naegleria fowleri and their role in contact-independent pathogenic mechanisms.Parasites & vectors · 2025Article
- Cloning of nf-profilin and intercellular interaction with nf-actin in Naegleria fowleri cysts.Scientific reports · 2025Article
- Extracellular Vesicles fromPathogens (Basel, Switzerland) · 2022Article
- Naegleria fowleri Induces Jurkat T Cell Death via O-deGlcNAcylation.The Korean journal of parasitology · 2021Article
- Differential Exoproteome and Biochemical Characterisation ofMicroorganisms · 2021Article
- Fowlerstefin, a cysteine protease inhibitor of Naegleria fowleri, induces inflammatory responses in BV-2 microglial cells in vitro.Parasites & vectors · 2020Article
- In Vitro Activity of Statins againstPathogens (Basel, Switzerland) · 2019Article
- Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts.The Korean journal of parasitology · 2019Article
- Naegleria fowleri after 50 years: is it a neglected pathogen?Journal of medical microbiology · 2016Article
- NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri.Infection and immunity · 2016Article
- Novel cathepsin B and cathepsin B-like cysteine protease of Naegleria fowleri excretory-secretory proteins and their biochemical properties.Parasitology research · 2014Article
- Naegleria fowleri lysate induces strong cytopathic effects and pro-inflammatory cytokine release in rat microglial cells.The Korean journal of parasitology · 2011Article
- Immunodominant antigens in Naegleria fowleri excretory--secretory proteins were potential pathogenic factors.Parasitology research · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Free-living Naegleria fowleri leads to a fatal infection known as primary amebic meningoencephalitis in humans. Previously, the target cell death could be induced by phagocytic activity of N. fowleri as a contact-dependent mechanism. However, in this study we investigated the target cell death under a non-contact system using a tissue-culture insert. The human microglial cells, U87MG cells, co-cultured with N. fowleri trophozoites for 30 min in a non-contact system showed morphological changes such as the cell membrane destruction and a reduction in the number. By fluorescence-activated cell sorter (FACS) analysis, U87MG cells co-cultured with N. fowleri trophozoites in a non-contact system showed a significant increase of apoptotic cells (16%) in comparison with that of the control or N. fowleri lysate. When U87MG cells were co-cultured with N. fowleri trophozoites in a non-contact system for 30 min, 2 hr, and 4 hr, the cytotoxicity of amebae against target cells was 40.5, 44.2, and 45.6%, respectively. By contrast, the cytotoxicity of non-pathogenic N. gruberi trophozoites was 10.2, 12.4, and 13.2%, respectively. These results suggest that the molecules released from N. fowleri in a contact-independent manner as well as phagocytosis in a contact-dependent manner may induce the host cell death.
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