ArticleJournal of cell science2009
Regulation of Rap1 activity is required for differential adhesion, cell-type patterning and morphogenesis in Dictyostelium.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- The Proteome ofProteomes · 2026Article
- Mutual suppression between mutations in the Dictyostelium Greenbeard pathway restores wild-type development.BMC genomics · 2025Article
- The IQGAP-related RasGAP IqgC regulates cell-substratum adhesion in Dictyostelium discoideum.Cellular & molecular biology letters · 2025Article
- Going against the family: Perturbation of a greenbeard pathway leads to falsebeard cheating.iScience · 2024Article
- Prestalk-like positioning of de-differentiated cells in the social amoeba Dictyostelium discoideum.Scientific reports · 2024Article
- ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation.FEBS open bio · 2022Article
- Adhesion ofFrontiers in cell and developmental biology · 2022Review
- Conditional expression explains molecular evolution of social genes in a microbe.Nature communications · 2019Article
- Cell Cycle Heterogeneity Can Generate Robust Cell Type Proportioning.Developmental cell · 2018Article
- The Saposin-Like Protein AplD Displays Pore-Forming Activity and Participates in Defense Against Bacterial Infection During a Multicellular Stage ofFrontiers in cellular and infection microbiology · 2018Article
- Progress and perspectives in signal transduction, actin dynamics, and movement at the cell and tissue level: lessons fromInterface focus · 2016Review
- Gene discovery by chemical mutagenesis and whole-genome sequencing in Dictyostelium.Genome research · 2016Article
- Direct Interaction between TalinB and Rap1 is necessary for adhesion of Dictyostelium cells.BMC cell biology · 2016Article
- RAP1-mediated MEK/ERK pathway defects in Kabuki syndrome.The Journal of clinical investigation · 2015Article
- Absence of catalytic domain in a putative protein kinase C (PkcA) suppresses tip dominance in Dictyostelium discoideum.Developmental biology · 2015Article
- Genetic control of morphogenesis in Dictyostelium.Developmental biology · 2015Review
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- The I/LWEQ domain in RapGAP3 required for posterior localization in migrating cells.Molecules and cells · 2014Article
- Mef2A, a homologue of animal Mef2 transcription factors, regulates cell differentiation in Dictyostelium discoideum.BMC developmental biology · 2013Article
- GxcC connects Rap and Rac signaling during Dictyostelium development.BMC cell biology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Regulated cell adhesion and motility have important roles during growth, development and tissue homeostasis. Consequently, great efforts have been made to identify genes that control these processes. One candidate is Rap1, as it has been implicated in the regulation of adhesion and motility in cell culture. To further study the role of Rap1 during multicellular development, we generated a mutant in a potential Rap1 GTPase activating protein (RapGAPB) in Dictyostelium. rapGAPB(-) cells have increased levels of active Rap1 compared with wild-type cells, indicating that RapGAPB regulates Rap1 activity. Furthermore, rapGAPB(-) cells exhibit hallmark phenotypes of other known mutants with hyperactivated Rap1, including increased substrate adhesion and abnormal F-actin distribution. However, unlike these other mutants, rapGAPB(-) cells do not exhibit impaired motility or chemotaxis, indicating that RapGAPB might only regulate specific roles of Rap1. Importantly, we also found that RapGAPB regulates Rap1 activity during multicellular development and is required for normal morphogenesis. First, streams of aggregating rapGAPB(-) cells break up as a result of decreased cell-cell adhesion. Second, rapGAPB(-) cells exhibit cell-autonomous defects in prestalk cell patterning. Using cell-type-specific markers, we demonstrate that RapGAPB is required for the correct sorting behaviour of different cell types. Finally, we show that inactivation of RapGAPB affects prestalk and prespore cell adhesion. We therefore propose that a possible mechanism for RapGAPB-regulated cell sorting is through differential adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.