ArticleJournal of cell science2009
Spontaneous phosphoinositide 3-kinase signaling dynamics drive spreading and random migration of fibroblasts.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 86 citations in OpenAlex.
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- A phase field model with stochastic input simulates cellular gradient sensing, morphodynamics, and fidelity of haptotaxis.bioRxiv : the preprint server for biology · 2026Article
- Substrate elasticity controls fibroblast motility on non-oxidized PDMS under weak adhesion.Biophysics and physicobiology · 2026Article
- Targeting Signaling Excitability in Cervical and Pancreatic Cancer Cells Through Combined Inhibition of FAK and PI3K.International journal of molecular sciences · 2025Article
- Ultra-low content physio-chemically crosslinked gelatin hydrogel improves encapsulated 3D cell culture.International journal of biological macromolecules · 2024Article
- Semi-autonomous wound invasion via matrix-deposited, haptotactic cues.Journal of theoretical biology · 2023Article
- Mechanosensitive stem cell fate choice is instructed by dynamic fluctuations in activation of Rho GTPases.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Microfluidic devices fitted with "flowver" paper pumps generate steady, tunable gradients for extended observation of chemotactic cell migration.Biomicrofluidics · 2021Article
- In Situ Subcellular Detachment of Cells Using a Cell-Friendly Photoresist and Spatially Modulated Light.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Excitable dynamics of Ras triggers spontaneous symmetry breaking of PIP3 signaling in motile cells.Journal of cell science · 2019Article
- Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation.Nature communications · 2018Article
- Live imaging reveals distinct modes of neutrophil and macrophage migration within interstitial tissues.Journal of cell science · 2017Article
- Mechanochemical feedback underlies coexistence of qualitatively distinct cell polarity patterns within diverse cell populations.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A mathematical model coupling polarity signaling to cell adhesion explains diverse cell migration patterns.PLoS computational biology · 2017Article
- ERK signaling promotes cell motility by inducing the localization of myosin 1E to lamellipodial tips.The Journal of cell biology · 2016Article
- Directed migration of cancer cells guided by the graded texture of the underlying matrix.Nature materials · 2016Article
- Article
- Time series modeling of live-cell shape dynamics for image-based phenotypic profiling.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Fibroblasts Lead the Way: A Unified View of 3D Cell Motility.Trends in cell biology · 2015Review
- Adhesion to fibronectin regulates Hippo signaling via the FAK-Src-PI3K pathway.The Journal of cell biology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
During directed cell migration (chemotaxis), cytoskeletal dynamics are stimulated and spatially biased by phosphoinositide 3-kinase (PI3K) and other signal transduction pathways. Live-cell imaging using total internal reflection fluorescence (TIRF) microscopy revealed that, in the absence of soluble cues, 3'-phosphoinositides are enriched in a localized and dynamic fashion during active spreading and random migration of mouse fibroblasts on adhesive surfaces. Surprisingly, we found that PI3K activation is uncoupled from classical integrin-mediated pathways and feedback from the actin cytoskeleton. Inhibiting PI3K significantly impairs cell motility, both in the context of normal spreading and when microtubules are dissociated, which induces a dynamic protrusion phenotype as seen by TIRF in our cells. Accordingly, during random migration, 3'-phosphoinositides are frequently localized to regions of membrane protrusion and correlate quantitatively with the direction and persistence of cell movement. These results underscore the importance of localized PI3K signaling not only in chemotaxis but also in basal motility/migration of fibroblasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.