ArticleJournal of neuroscience research2009
A novel method for isolating Schwann cells using the extracellular domain of Necl1.
Article in Journal of neuroscience research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- A practical CD90.2-based FACS strategy for obtaining Schwann cell-enriched cultures from adult mouse nerves.Frontiers in cellular neuroscience · 2026Article
- The Current Challenges for Drug Discovery in CNS Remyelination.International journal of molecular sciences · 2021Review
- Evaluation of two methods to isolate Schwann cells from murine sciatic nerve.Journal of neuroscience methods · 2020Article
- Automated image analysis of stained cytospins to quantify Schwann cell purity and proliferation.PloS one · 2020Article
- Isolation of rat Schwann cells based on cell sorting.Molecular medicine reports · 2017Article
- Primary culture of human Schwann and schwannoma cells: improved and simplified protocol.Hearing research · 2014Article
- Direct genesis of functional rodent and human schwann cells from skin mesenchymal precursors.Stem cell reports · 2014Article
- A culture system to study oligodendrocyte myelination processes using engineered nanofibers.Nature methods · 2012Article
- Functional dissection of the Oct6 Schwann cell enhancer reveals an essential role for dimeric Sox10 binding.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Myelinating cocultures of Schwann cells and dorsal root ganglion neurons are a powerful experimental system for probing the molecular mechanisms of axon-Schwann cell interaction. The isolation of a pure population of myelination-competent Schwann cells is a prerequisite for this experimental system. We describe here a protocol for a FACS-based isolation of Schwann cells utilizing a specific affinity reagent (Necl1-Fc) and the use of these isolated cells in myelinating cocultures. An advantage of the myelinating coculture system is that Schwann cells and the neurons can be genetically manipulated before they are cocultured. We further show that our method allows the isolation of virally transduced Schwann cells in a single purification step. This protocol for the FACS-based isolation of myelination-competent Schwann cells by Necl1-Fc and the use of these cells in myelinating cocultures should significantly facilitate future studies aimed at delineation of the molecular mechanisms of axon-Schwann cell interactions and myelination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.