ArticleMolecular and cellular biology2009
Preimplantation mouse embryos depend on inhibitory phosphorylation of separase to prevent chromosome missegregation.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Orchestrating homolog segregation in meiosis I: molecular logic and regulatory networks with emphasis on male metaphase I.Cell communication and signaling : CCS · 2026Review
- SGO2 does not play an essential role in separase inhibition during meiosis I in mouse oocytes.PLoS biology · 2025Article
- Separase and Roads to Disengage Sister Chromatids during Anaphase.International journal of molecular sciences · 2023Review
- Review
- Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies.Frontiers in endocrinology · 2022Review
- Impact of Global Transcriptional Silencing on Cell Cycle Regulation and Chromosome Segregation in Early Mammalian Embryos.International journal of molecular sciences · 2021Review
- Article
- Structure and Function of the Separase-Securin Complex.Sub-cellular biochemistry · 2021Review
- Intertwined Functions of Separase and Caspase in Cell Division and Programmed Cell Death.Scientific reports · 2020Article
- Article
- Overexpression of ubiquitin specific proteases 44 promotes the malignancy of glioma by stabilizing tumor-promoter securin.Oncotarget · 2017Article
- The ubiquitin ligase CRL2ZYG11 targets cyclin B1 for degradation in a conserved pathway that facilitates mitotic slippage.The Journal of cell biology · 2016Article
- Positive and negative regulation of vertebrate separase by Cdk1-cyclin B1 may explain why securin is dispensable.The Journal of biological chemistry · 2015Article
- Overexpression of a truncated CTF7 construct leads to pleiotropic defects in reproduction and vegetative growth in Arabidopsis.BMC plant biology · 2015Article
- PP2A delays APC/C-dependent degradation of separase-associated but not free securin.The EMBO journal · 2014Article
- Protein phosphatase 5 is a negative regulator of separase function during cortical granule exocytosis in C. elegans.Journal of cell science · 2011Article
- The sister bonding of duplicated chromosomes.Seminars in cell & developmental biology · 2011Review
- Separase phosphosite mutation leads to genome instability and primordial germ cell depletion during oogenesis.PloS one · 2011Article
- DNA-dependent cohesin cleavage by separase.Nucleus (Austin, Tex.)Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Separase is a critical protease that catalyzes the cleavage of sister chromatid cohesins to allow the separation of sister chromatids in the anaphase. Its activity must be inhibited prior to the onset of the anaphase. Two inhibitory mechanisms exist in vertebrates that block the protease activity. One mechanism is through binding and inhibition by securin, and another is phosphorylation on Ser1126 (in humans [Ser1121 in mice]). These two mechanisms are largely redundant. However, phosphorylation on Ser1121 is critical for the prevention of premature sister separation in embryonic germ cells. As a result, Ser1121-to-Ala mutation leads to depletion of germ cells in development and subsequently to infertility in mice. Here, we report that the same mutation also causes embryogenesis failure between the 8- and 16-cell stages in mice. Our results indicate a critical role of separase phosphorylation in germ cell development as well as in early embryogenesis. Thus, deregulation of separase may be a significant contributor to infertility in humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.