ArticleInfection and immunity2009
Anthrax lethal toxin triggers the formation of a membrane-associated inflammasome complex in murine macrophages.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 83 citations in OpenAlex.
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- Redundant role of PAD2 and PAD4 in the development of cardiovascular lesions in a mouse model of Kawasaki disease vasculitis.Clinical and experimental immunology · 2024Article
- Regulation of NLRP3 Inflammasome Activation and Inflammatory Exosome Release in Podocytes by Acid Sphingomyelinase During Obesity.Inflammation · 2023Article
- Ubiquitination as a key regulatory mechanism for ORedox biology · 2022Article
- Contribution of podocyte inflammatory exosome release to glomerular inflammation and sclerosis during hyperhomocysteinemia.Biochimica et biophysica acta. Molecular basis of disease · 2021Article
- Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes.Redox biology · 2021Article
- NLRP12 in innate immunity and inflammation.Molecular aspects of medicine · 2020Review
- ATP-Binding and Hydrolysis in Inflammasome Activation.Molecules (Basel, Switzerland) · 2020Review
- A comprehensive guide to studying inflammasome activation and cell death.Nature protocols · 2020Article
- NLRP1 and NLRP3 inflammasomes as a new approach to skin carcinogenesis.Oncology letters · 2020Review
- Blocking transmembrane219 protein signaling inhibits autophagy and restores normal cell death.PloS one · 2019Article
- Inflammasome activation and assembly at a glance.Journal of cell science · 2017Review
- Inflammasomes and intestinal inflammation.Mucosal immunology · 2017Review
- ASC filament formation serves as a signal amplification mechanism for inflammasomes.Nature communications · 2016Article
- The cell biology of inflammasomes: Mechanisms of inflammasome activation and regulation.The Journal of cell biology · 2016Review
- The effect of PEGylation on the stimulation of IL-1β by gold (Au) nanoshell/silica core nanoparticles.Journal of materials chemistry. B · 2016Article
- Mechanisms of inflammasome activation: recent advances and novel insights.Trends in cell biology · 2015Review
- Beyond canonical inflammasomes: emerging pathways in IL-1-mediated autoinflammatory disease.Seminars in immunopathology · 2014Review
- Inflammasome sensor NLRP1 controls rat macrophage susceptibility to Toxoplasma gondii.PLoS pathogens · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Multiple microbial components trigger the formation of an inflammasome complex that contains pathogen-specific nucleotide oligomerization and binding domain (NOD)-like receptors (NLRs), caspase-1, and in some cases the scaffolding protein ASC. The NLR protein Nalp1b has been linked to anthrax lethal toxin (LT)-mediated cytolysis of murine macrophages. Here we demonstrate that in unstimulated J774A.1 macrophages, caspase-1 and Nalp1b are membrane associated and part of approximately 200- and approximately 800-kDa complexes, respectively. LT treatment of these cells resulted in caspase-1 recruitment to the Nalp1b-containing complex, concurrent with processing of cytosolic caspase-1 substrates. We further demonstrated that Nalp1b and caspase-1 are able to interact with each other. Intriguingly, both caspase-1 and Nalp1b were membrane associated, while the caspase-1 substrate interleukin-18 was cytosolic. Caspase-1-associated inflammasome components included, besides Nalp1b, proinflammatory caspase-11 and the caspase-1 substrate alpha-enolase. Asc was not part of the Nalp1b inflammasome in LT-treated macrophages. Taken together, our findings suggest that LT triggers the formation of a membrane-associated inflammasome complex in murine macrophages, resulting in cleavage of cytosolic caspase-1 substrates and cell death.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.