ArticleBiophysical journal1991
Spatial Fourier analysis of video photobleaching measurements. Principles and optimization.
Article in Biophysical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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Who cites it
45 citing papers in PubMed, 88 citations in OpenAlex.
- Human mesenchymal stem/stromal cell-derived extracellular vesicle transport in meniscus fibrocartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Assessing the role of surface layer and molecular probe size in diffusion within meniscus tissue.PloS one · 2024Article
- Review
- High-Throughput FRAP Analysis of Solute Diffusion in Hydrogels.Macromolecules · 2021Article
- A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion.Nature communications · 2021Article
- Defining the Diffusion in Model Membranes Using Line Fluorescence Recovery after Photobleaching.Membranes · 2020Article
- EFFECTS OF SOLUTE SIZE AND TISSUE COMPOSITION ON MOLECULAR AND MACROMOLECULAR DIFFUSIVITY IN HUMAN KNEE CARTILAGE.Osteoarthritis and cartilage open · 2020Article
- Anomalous Diffusion Characterization by Fourier Transform-FRAP with Patterned Illumination.Biophysical journal · 2020Article
- Photoactivatable fluorescent probes reveal heterogeneous nanoparticle permeation through biological gels at multiple scales.Journal of controlled release : official journal of the Controlled Release Society · 2017Article
- Single- and two-photon fluorescence recovery after photobleaching.Cold Spring Harbor protocols · 2015Article
- Measurement of three-dimensional anisotropic diffusion by multiphoton fluorescence recovery after photobleaching.Annals of biomedical engineering · 2014Article
- FRAP in pharmaceutical research: practical guidelines and applications in drug delivery.Pharmaceutical research · 2014Review
- Analysis of vaginal microbicide film hydration kinetics by quantitative imaging refractometry.PloS one · 2014Article
- A nonfitting method using a spatial sine window transform for inhomogeneous effective-diffusion measurements by FRAP.Biophysical journal · 2011Article
- Simultaneous measurement of anisotropic solute diffusivity and binding reaction rates in biological tissues by FRAP.Annals of biomedical engineering · 2011Article
- Anisotropic solute diffusion tensor in porcine TMJ discs measured by FRAP with spatial Fourier analysis.Annals of biomedical engineering · 2010Article
- Relationship between solute transport properties and tissue morphology in human annulus fibrosus.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2009Article
- Capturing the nanoscale complexity of cellular membranes in supported lipid bilayers.Journal of structural biology · 2009Review
- Improved model of fluorescence recovery expands the application of multiphoton fluorescence recovery after photobleaching in vivo.Biophysical journal · 2009Article
- Convolution-based one and two component FRAP analysis: theory and application.European biophysics journal : EBJ · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The major use of the fluorescence recovery after photobleaching (FRAP) technique is to measure the translational motion of the molecular components in various condensed media. In a conventional laser spot photobleaching experiment, a photomultiplier is used to measure the total brightness levels of the bleached region in the sample, so no spatial information can be directly obtained. In video-FRAP, a series of images after photobleaching is acquired, allowing the spatial character of the recovery to be determined; this permits direct detection of both anisotropic diffusion and flow. To utilize all of the available image data to determine the transport coefficients, a two-dimensional spatial Fourier transform analysis of the images after photobleaching was employed. The change in the transform between two time points reflects the action of diffusion during the interim. An important advantage of this method, which involves taking the ratio of image transforms at different time points, is that it does not require a specific initial condition to be created by laser photobleaching. The ability of the analysis to extract transport coefficients from computer-simulated diffusional recovery is assessed in the presence of increasing amounts of noise. Experimental data analysis from the diffusion of proteins in viscous solutions and from the diffusion of protein receptors on cell surfaces demonstrate the feasibility of the Fourier analysis to obtain transport coefficients from the video FRAP measurement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.