ArticleThe Journal of biological chemistry2009
Characterization and in vitro reaction properties of 19 unique hairpin telomeres from the linear plasmids of the lyme disease spirochete.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- Protocol for telomere-to-telomere assembly of Borrelia genomes using a hybrid method.STAR protocols · 2026Article
- Identification of essential genes for conjugative transfer in antimicrobial resistance-associated pELF-type linear plasmids of opportunistic pathogen Enterococcus faecium.PLoS pathogens · 2026Article
- Latent endogenous giant viruses drive active infection and inheritance in a multicellular algal host.Nature microbiology · 2026Article
- Comparative genomics of Borrelia lusitaniae.G3 (Bethesda, Md.) · 2026Article
- The wild, wild west of plasmids: First insights into comparative genomics of Borrelia burgdorferi sensu lato.PloS one · 2026Article
- Whole-genome sequences of sixMicrobiology resource announcements · 2025Article
- Breaking a barrier: In trans vlsE recombination and genetic manipulation of the native vlsE gene of the Lyme disease pathogen.PLoS pathogens · 2025Article
- Structure analysis of the telomere resolvase from the Lyme disease spirochete Borrelia garinii reveals functional divergence of its C-terminal domain.Nucleic acids research · 2024Article
- Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1.PLoS pathogens · 2024Article
- Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1.bioRxiv : the preprint server for biology · 2024Article
- Genomic Confirmation of Borrelia garinii, United States.Emerging infectious diseases · 2023Article
- The telomere resolvase, TelA, utilizes an underwound pre-cleavage intermediate to promote hairpin telomere formation.PloS one · 2023Article
- Changing of the guard: How the Lyme disease spirochete subverts the host immune response.The Journal of biological chemistry · 2020Review
- Whole genome sequencing of Borrelia miyamotoi isolate Izh-4: reference for a complex bacterial genome.BMC genomics · 2020Article
- Primordial origin and diversification of plasmids in Lyme disease agent bacteria.BMC genomics · 2018Article
- Plasmid diversity and phylogenetic consistency in the Lyme disease agent Borrelia burgdorferi.BMC genomics · 2017Article
- Spring loading a pre-cleavage intermediate for hairpin telomere formation.Nucleic acids research · 2015Article
- Article
- Construction and characterization of a Borrelia burgdorferi strain with conditional expression of the essential telomere resolvase, ResT.Journal of bacteriology · 2014Article
- Presence of Arp specifically contributes to joint tissue edema associated with early-onset Lyme arthritis.Infection and immunity · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The genome of the Lyme disease pathogen Borrelia burgdorferi contains about a dozen linear DNA molecules that carry covalently closed hairpin telomeres as a specialized mechanism for dealing with the end-replication problem. The hairpin telomeres are generated from replicative intermediates through a two-step transesterification promoted by the telomere resolvase ResT. Although the genome of B. burgdorferi has been sequenced, the sequence of most telomeres has remained unknown because of difficulties in recovering and completely sequencing the covalently closed hairpin ends. In this study we report a new approach for the direct sequencing Borrelia telomeres and report the sequence, characterization, and in vitro reaction properties of 19 unique telomeres. Surprisingly, a variation of greater than 160-fold in the initial reaction rates of in vitro ResT-mediated telomere resolution was observed between the most active and least active telomeres. Moreover, three of the hairpin telomeres were completely inactive in vitro, but their in vivo functionality was demonstrated. Our results provide important new information on the structure and function of the B. burgdorferi telomeres and suggest the possibility that factors besides the telomere resolvase ResT may influence the reaction in vivo and rescue those telomeres that are not functional in vitro with ResT alone.
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