ArticleArteriosclerosis, thrombosis, and vascular biology2009
Fibrinogen-gamma C-terminal fragments induce endothelial barrier dysfunction and microvascular leak via integrin-mediated and RhoA-dependent mechanism.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Fibrinogen as a Driver of Oxidative Stress and Apoptosis in the Fetal Brain After Maternal Influenza.Research square · 2026Article
- Focal adhesion kinase mediates microvascular leakage and endothelial barrier dysfunction in ischemia-reperfusion injury.Microvascular research · 2025Article
- Calpain Promotes LPS-induced Lung Endothelial Barrier Dysfunction via Cleavage of Talin.American journal of respiratory cell and molecular biology · 2023Article
- Are Differences in Inflammatory Markers between Patients with and without Hypertension-Mediated Organ Damage Influenced by Circadian Blood Pressure Abnormalities?Journal of clinical medicine · 2022Article
- The role of exogenous Fibrinogen in cardiac surgery: stop bleeding or induce cardiovascular disease.Molecular biology reports · 2020Review
- Article
- Focal adhesion kinase and Src mediate microvascular hyperpermeability caused by fibrinogen- γC- terminal fragments.PloS one · 2020Article
- Epithelial to Mesenchymal transition, eIF2α phosphorylation and Hsp70 expression enable greater tolerance in A549 cells to TiOScientific reports · 2019Article
- Role of Neutrophil Extracellular Traps and Vesicles in Regulating Vascular Endothelial Permeability.Frontiers in immunology · 2019Review
- Significance of glomerular fibrinogen deposition in children with Henoch-Schönlein purpura nephritis.Italian journal of pediatrics · 2018Article
- Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery.Journal of visualized experiments : JoVE · 2015Article
- Blood cells and endothelial barrier function.Tissue barriers · 2015Article
- Neutrophil transmigration, focal adhesion kinase and endothelial barrier function.Microvascular research · 2012Review
- Serum metalloproteinases MMP-2, MMP-9, and metalloproteinase tissue inhibitors in patients are associated with arteriovenous fistula maturation.Journal of vascular surgery · 2011Article
- Alzheimer's disease peptide beta-amyloid interacts with fibrinogen and induces its oligomerization.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Vein tissue expression of matrix metalloproteinase as biomarker for hemodialysis arteriovenous fistula maturation.Vascular and endovascular surgery · 2010Article
- Tissue inhibitor of metalloproteinase-2 regulates matrix metalloproteinase-2-mediated endothelial barrier dysfunction and breast cancer cell transmigration through lung microvascular endothelial cells.Molecular cancer research : MCR · 2010Article
- Mechanisms of fibrinogen-induced microvascular dysfunction during cardiovascular disease.Acta physiologica (Oxford, England) · 2010Review
- Endothelial contractile cytoskeleton and microvascular permeability.Cell health and cytoskeleton · 2009Article
- Fibrin Degradation Products Regulate Endothelial Barrier Function in Traumatic Brain Injury via the Actin Cytoskeleton.Neurotrauma reportsArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
objectivesThe purposes of this study were to characterize the direct effect of the C-terminal fragment of fibrinogen gamma chain (gammaC) on microvascular endothelial permeability and to examine its molecular mechanism of action. METHODS AND
resultsIntravital microscopy was performed to measure albumin extravasation in intact mesenteric microvasculature, followed by quantification of hydraulic conductivity in single perfused microvessels. Transendothelial electric resistance was measured in microvascular endothelial cells in combination with immunoblotting and immunocytochemistry. The results show that gammaC induced time- and concentration-dependent increases in protein transvascular flux and water permeability and decreases in endothelial barrier function, coupled with Rho GTPase activation, myosin light chain phosphorylation, and stress fiber formation. Depletion of RhoA via siRNA knockdown or pharmacological inhibition of RhoA signaling attenuated gammaC-induced barrier dysfunction. Imaging analyses demonstrated binding of gammaC to endothelial cells; the interaction was inhibited during blockage of the alphavbeta3 integrin. Furthermore, in vivo experiments showed that the microvascular leak response to gammaC was attenuated in integrin beta3(-/-) animals.
conclusionFibrinogen-gamma C terminus directly interacts with the microvascular endothelium causing fluid and protein leak. The endothelial response to gammaC involves an integrin receptor-mediated RhoA-dependent signaling pathway that leads to paracellular hyperpermeability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.