ArticleThe Plant cell2009
A polypyrimidine tract binding protein, pumpkin RBP50, forms the basis of a phloem-mobile ribonucleoprotein complex.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
86 citing papers in PubMed, 205 citations in OpenAlex.
- Transcription factors in phosphorus utilization: enhancing crop productivity and stress resilience.Planta · 2026Review
- Small RNAs as systemic signals in plant defense: mechanisms, challenges, and future directions.Molecular biology reports · 2026Review
- Genome Editing by Grafting.International journal of molecular sciences · 2025Review
- Umbravirus-like RNA viruses are capable of independent systemic plant infection in the absence of encoded movement proteins.PLoS biology · 2024Article
- Evolutionary and Structural Analysis of PP16 in Viridiplantae.International journal of molecular sciences · 2024Article
- Arabidopsis cyclophilins direct intracellular transport of mobile mRNA via organelle hitchhiking.Nature plants · 2024Article
- BEL transcription factors in prominent Solanaceae crops: the missing pieces of the jigsaw in plant development.Planta · 2023Review
- Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato.International journal of molecular sciences · 2023Article
- Phloem-MobilePlants (Basel, Switzerland) · 2023Article
- Systemic movement of long non-coding RNA ELENA1 attenuates leaf senescence under nitrogen deficiency.Nature plants · 2023Article
- Plasmodesmal endoplasmic reticulum proteins regulate intercellular trafficking of cucumber mosaic virus in Arabidopsis.Journal of experimental botany · 2023Article
- Toward a systems view on RNA-binding proteins and associated RNAs in plants: Guilt by association.The Plant cell · 2023Article
- The mRNA mobileome: challenges and opportunities for deciphering signals from the noise.The Plant cell · 2023Review
- The β-1,3-Glucanase Degrades Callose at Plasmodesmata to Facilitate the Transport of the Ribonucleoprotein Complex inInternational journal of molecular sciences · 2023Article
- Large-scale mRNA transfer betweeniScience · 2023Article
- Long distance signalling and epigenetic changes in crop grafting.Frontiers in plant science · 2023Review
- CUCUME: An RNA methylation database integrating systemic mRNAs signals, GWAS and QTL genetic regulation and epigenetics in different tissues ofComputational and structural biotechnology journal · 2023Article
- Transcriptomic alterations in the sweet orange vasculature correlate with growth repression induced by a variant of citrus tristeza virus.Frontiers in microbiology · 2023Article
- Long-distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization.EMBO reports · 2022Article
- Review
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA binding proteins (RBPs) are integral components of ribonucleoprotein (RNP) complexes and play a central role in RNA processing. In plants, some RBPs function in a non-cell-autonomous manner. The angiosperm phloem translocation stream contains a unique population of RBPs, but little is known regarding the nature of the proteins and mRNA species that constitute phloem-mobile RNP complexes. Here, we identified and characterized a 50-kD pumpkin (Cucurbita maxima cv Big Max) phloem RNA binding protein (RBP50) that is evolutionarily related to animal polypyrimidine tract binding proteins. In situ hybridization studies indicated a high level of RBP50 transcripts in companion cells, while immunolocalization experiments detected RBP50 in both companion cells and sieve elements. A comparison of the levels of RBP50 present in vascular bundles and phloem sap indicated that this protein is highly enriched in the phloem sap. Heterografting experiments confirmed that RBP50 is translocated from source to sink tissues. Collectively, these findings established that RBP50 functions as a non-cell-autonomous RBP. Protein overlay, coimmunoprecipitation, and cross-linking experiments identified the phloem proteins and mRNA species that constitute RBP50-based RNP complexes. Gel mobility-shift assays demonstrated that specificity, with respect to the bound mRNA, is established by the polypyrimidine tract binding motifs within such transcripts. We present a model for RBP50-based RNP complexes within the pumpkin phloem translocation stream.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.