ArticleBMC microbiology2009
Large scale multiplex PCR improves pathogen detection by DNA microarrays.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 206 citations in OpenAlex.
- Quantitative Multiplex Digital PCR with Fluorescence-Encoded Nanoreactor Beads.Analytical chemistry · 2026Article
- Epidemiology of positive bacterial cultures and the coverage gaps in multiplex PCR diagnostics: a single-center retrospective study.Journal of clinical microbiology · 2025Article
- Molecular and serological incidences ofOpen veterinary journal · 2025Article
- ISCCM Position Statement on the Management of Invasive Fungal Infections in the Intensive Care Unit.Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine · 2024Article
- Expanded Multiplexing on Sensor-Constrained Microfluidic Partitioning Systems.Analytical chemistry · 2023Article
- Diagnosis of invasive fungal infections: challenges and recent developments.Journal of biomedical science · 2023Review
- Novel next generation sequencing panel method for the multiple detection and identification of foodborne pathogens in agricultural wastewater.Frontiers in microbiology · 2023Article
- Bibliometric analysis of real-time PCR-based pathogen detection in plant protection research: a comprehensive study.Frontiers in plant science · 2023Article
- Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality.International journal of environmental research and public health · 2022Review
- Application of Engineered Zinc Finger Proteins Immobilized on Paramagnetic Beads for Multiplexed Detection of Pathogenic DNA.Journal of microbiology and biotechnology · 2021Article
- CovidArray: A Microarray-Based Assay with High Sensitivity for the Detection of Sars-Cov-2 in Nasopharyngeal Swabs.Sensors (Basel, Switzerland) · 2021Article
- Advances in Metagenomics and Its Application in Environmental Microorganisms.Frontiers in microbiology · 2021Review
- Manipulation of the Gut Microbiome Alters Acetaminophen Biodisposition in Mice.Scientific reports · 2020Article
- Molecular approaches to the diagnosis and monitoring of production diseases in pigs.Research in veterinary science · 2017Review
- Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection.Clinical chemistry · 2017Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
- The development and application of a molecular community profiling strategy to identify polymicrobial bacterial DNA in the whole blood of septic patients.BMC microbiology · 2015Article
- Solid and Suspension Microarrays for Microbial Diagnostics.Methods in microbiology · 2015Article
- Optical and dielectric sensors based on antimicrobial peptides for microorganism diagnosis.Frontiers in microbiology · 2014Review
- Challenges and solutions in the development of genomic biomarker panels: a systematic phased approach.Current genomics · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMedium density DNA microchips that carry a collection of probes for a broad spectrum of pathogens, have the potential to be powerful tools for simultaneous species identification, detection of virulence factors and antimicrobial resistance determinants. However, their widespread use in microbiological diagnostics is limited by the problem of low pathogen numbers in clinical specimens revealing relatively low amounts of pathogen DNA.
resultsTo increase the detection power of a fluorescence-based prototype-microarray designed to identify pathogenic microorganisms involved in sepsis, we propose a large scale multiplex PCR (LSplex PCR) for amplification of several dozens of gene-segments of 9 pathogenic species. This protocol employs a large set of primer pairs, potentially able to amplify 800 different gene segments that correspond to the capture probes spotted on the microarray. The LSplex protocol is shown to selectively amplify only the gene segments corresponding to the specific pathogen present in the analyte. Application of LSplex increases the microarray detection of target templates by a factor of 100 to 1000.
conclusionOur data provide a proof of principle for the improvement of detection of pathogen DNA by microarray hybridization by using LSplex PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.