ArticleJournal of cellular and molecular medicine2009
Structural and mechanistic insights into Mps1 kinase activation.
Article in Journal of cellular and molecular medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
- MPS1 inhibition as an anticancer strategy: where do we stand?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Review
- Chemical Genetics with SP600125 Reveals That Mps1 Protein Kinase Works as a Regulatory Element in Post-embryonic Development of theACS omega · 2025Article
- Combined 3D-QSAR, molecular docking and dynamics simulations studies to model and design TTK inhibitors.Frontiers in chemistry · 2022Article
- The essential roles of Mps1 in spermatogenesis and fertility in mice.Cell death & disease · 2021Article
- Phosphatases in Mitosis: Roles and Regulation.Biomolecules · 2019Review
- Article
- Leader of the SAC: molecular mechanisms of Mps1/TTK regulation in mitosis.Open biology · 2018Review
- Mps1 Phosphorylates Its N-Terminal Extension to Relieve Autoinhibition and Activate the Spindle Assembly Checkpoint.Current biology : CB · 2018Article
- Mechanisms of Mitotic Kinase Regulation: A Structural Perspective.Frontiers in cell and developmental biology · 2018Review
- Mps1 is SUMO-modified during the cell cycle.Oncotarget · 2016Article
- Article
- Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function.Molecular biology of the cell · 2015Article
- TTK activates Akt and promotes proliferation and migration of hepatocellular carcinoma cells.Oncotarget · 2015Article
- Mitosis as an anti-cancer drug target.Chromosoma · 2013Review
- On the molecular mechanisms of mitotic kinase activation.Open biology · 2012Review
- Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1.Molecular biology of the cell · 2012Article
- The MPS1 family of protein kinases.Annual review of biochemistry · 2012Review
- Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana.PloS one · 2012Article
- Mip1 associates with both the Mps1 kinase and actin, and is required for cell cortex stability and anaphase spindle positioning.Cell cycle (Georgetown, Tex.) · 2011Article
- Cellular abundance of Mps1 and the role of its carboxyl terminal tail in substrate recruitment.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Mps1 is one of the several essential kinases whose activation is required for robust mitotic spindle checkpoint signalling. The activity of Mps1 is tightly regulated and increases dramatically during mitosis or in response to spindle damage. To understand the molecular mechanism underlying Mps1 regulation, we determined the crystal structure of the kinase domain of Mps1. The 2.7-A-resolution crystal structure shows that the Mps1 kinase domain adopts a unique inactive conformation. Intramolecular interactions between the key Glu residue in the C helix of the N-terminal lobe and the backbone amides in the catalytic loop lock the kinase in the inactive conformation. Autophosphorylation appears to be a priming event for kinase activation. We identified Mps1 autophosphorylation sites in the activation and the P+1 loops. Whereas activation loop autophosphorylation enhances kinase activity, autophosphorylation at the P+1 loop (T686) is associated with the active kinase. Mutation of T686 autophosphorylation site impairs both autophosphorylation and transphosphorylation. Furthermore, we demonstrated that phosphorylation of T676 may be a priming event for phosphorylation at T686. Finally, we identified two critical lysine residues in the loop between helices EF and F that are essential for substrate recruitment and maintaining high levels of kinase activity. Our studies reveal critical biochemical mechanisms for Mps1 kinase regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.