ArticlePloS one2009
Display of cell surface sites for fibronectin assembly is modulated by cell adherence to (1)F3 and C-terminal modules of fibronectin.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A disintegrin-like and metalloproteinase domain with thrombospondin type 1 motif 9 (ADAMTS9) regulates fibronectin fibrillogenesis and turnover.The Journal of biological chemistry · 2019Article
- Fibronectin promotes directional persistence in fibroblast migration through interactions with both its cell-binding and heparin-binding domains.Scientific reports · 2017Article
- Bivalent ligation of the collagen-binding modules of fibronectin by SFS, a non-anchored bacterial protein of Streptococcus equi.The Journal of biological chemistry · 2015Article
- Borrelia burgdorferi protein BBK32 binds to soluble fibronectin via the N-terminal 70-kDa region, causing fibronectin to undergo conformational extension.The Journal of biological chemistry · 2014Article
- Opposing effects of collagen I and vitronectin on fibronectin fibril structure and function.Matrix biology : journal of the International Society for Matrix Biology · 2014Article
- Fibronectin matrix mimetics promote full-thickness wound repair in diabetic mice.Tissue engineering. Part A · 2013Article
- The fibronectin-binding motif within FlpA facilitates Campylobacter jejuni adherence to host cell and activation of host cell signaling.Emerging microbes & infections · 2013Article
- Recombinant fibronectin matrix mimetics specify integrin adhesion and extracellular matrix assembly.Tissue engineering. Part A · 2013Article
- Secretomic analysis identifies alpha-1 antitrypsin (A1AT) as a required protein in cancer cell migration, invasion, and pericellular fibronectin assembly for facilitating lung colonization of lung adenocarcinoma cells.Molecular & cellular proteomics : MCP · 2012Article
- Quantitative microtiter fibronectin fibrillogenesis assay: use in high throughput screening for identification of inhibitor compounds.Matrix biology : journal of the International Society for Matrix Biology · 2012Article
- Ligation of the fibrin-binding domain by β-strand addition is sufficient for expansion of soluble fibronectin.The Journal of biological chemistry · 2012Article
- Surface derivatization strategy for combinatorial analysis of cell response to mixtures of protein domains.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Article
- Reactivity of the N-terminal region of fibronectin protein to transglutaminase 2 and factor XIIIA.The Journal of biological chemistry · 2011Article
- Homocysteine modifies structural and functional properties of fibronectin and interferes with the fibronectin-fibrillin-1 interaction.Biochemistry · 2011Article
- Extended binding site on fibronectin for the functional upstream domain of protein F1 of Streptococcus pyogenes.The Journal of biological chemistry · 2010Article
- iso-DGR sequences do not mediate binding of fibronectin N-terminal modules to adherent fibronectin-null fibroblasts.The Journal of biological chemistry · 2010Article
- Assembly of fibronectin extracellular matrix.Annual review of cell and developmental biology · 2010Review
- Fibronectin binds and enhances the activity of bone morphogenetic protein 1.The Journal of biological chemistry · 2009Article
- Motogenic sites in human fibronectin are masked by long range interactions.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundFibronectin-null cells assemble soluble fibronectin shortly after adherence to a substrate coated with intact fibronectin but not when adherent to the cell-binding domain of fibronectin (modules (7)F3-(10)F3). Interactions of adherent cells with regions of adsorbed fibronectin other than modules (7)F3-(10)F3, therefore, are required for early display of the cell surface sites that initiate and direct fibronectin assembly. METHODOLOGY/PRINCIPAL
findingsTo identify these regions, coatings of proteolytically derived or recombinant pieces of fibronectin containing modules in addition to (7)F3-(10)F3 were tested for effects on fibronectin assembly by adherent fibronectin-null fibroblasts. Pieces as large as one comprising modules (2)F3-(14)F3, which include the heparin-binding and cell adhesion domains, were not effective in supporting fibronectin assembly. Addition of module (1)F3 or the C-terminal modules to modules (2)F3-(14)F3 resulted in some activity, and addition of both (1)F3 and the C-terminal modules resulted in a construct, (1)F3-C, that best mimicked the activity of a coating of intact fibronectin. Constructs (1)F3-C V0, (1)F3-C V64, and (1)F3-C Delta(V(15)F3(10)F1) were all able to support fibronectin assembly, suggesting that (1)F3 through (11)F1 and/or (12)F1 were important for activity. Coatings in which the active parts of (1)F3-C were present in different proteins were much less active than intact (1)F3-C.
conclusionsThese results suggest that (1)F3 acts together with C-terminal modules to induce display of fibronectin assembly sites on adherent cells.
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