ArticleMicrobiology (Reading, England)2009
Experimental determination of translational start sites resolves uncertainties in genomic open reading frame predictions - application to Mycobacterium tuberculosis.
Article in Microbiology (Reading, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Real-time kinetic studies of Mycobacterium tuberculosis LexA-DNA interaction.Bioscience reports · 2021Article
- Reversible gene silencing through frameshift indels and frameshift scars provide adaptive plasticity for Mycobacterium tuberculosis.Nature communications · 2021Article
- StartLink and StartLink+: Prediction of Gene Starts in Prokaryotic Genomes.Frontiers in bioinformatics · 2021Review
- Structural insights into chaperone addiction of toxin-antitoxin systems.Nature communications · 2019Article
- Cell-wall synthesis and ribosome maturation are co-regulated by an RNA switch in Mycobacterium tuberculosis.Nucleic acids research · 2018Article
- Toxin-Antitoxin Systems in Clinical Pathogens.Toxins · 2016Review
- A Novel Quality Measure and Correction Procedure for the Annotation of Microbial Translation Initiation Sites.PloS one · 2015Article
- Comparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteins.BMC genomics · 2014Article
- The Streptococcus suis transcriptional landscape reveals adaptation mechanisms in pig blood and cerebrospinal fluid.RNA (New York, N.Y.) · 2014Article
- Review
- Induced ectopic expression of HigB toxin in Mycobacterium tuberculosis results in growth inhibition, reduced abundance of a subset of mRNAs and cleavage of tmRNA.Molecular microbiology · 2013Article
- Characterisation of the Mycobacterium tuberculosis alternative sigma factor SigG: its operon and regulon.Tuberculosis (Edinburgh, Scotland) · 2013Article
- TAC from Mycobacterium tuberculosis: a paradigm for stress-responsive toxin-antitoxin systems controlled by SecB-like chaperones.Cell stress & chaperones · 2013Article
- Reannotation of translational start sites in the genome of Mycobacterium tuberculosis.Tuberculosis (Edinburgh, Scotland) · 2013Article
- High-resolution transcriptome and genome-wide dynamics of RNA polymerase and NusA in Mycobacterium tuberculosis.Nucleic acids research · 2013Article
- Bioinformatic and empirical analysis of novel hypoxia-inducible targets of the human antituberculosis T cell response.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Global analysis of the regulon of the transcriptional repressor LexA, a key component of SOS response in Mycobacterium tuberculosis.The Journal of biological chemistry · 2012Article
- Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene.Nucleic acids research · 2011Article
- SecB-like chaperone controls a toxin-antitoxin stress-responsive system in Mycobacterium tuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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Authors and funding
6 authors.
Funding
Abstract
Correct identification of translational start sites is important for understanding protein function and transcriptional regulation. The annotated translational start sites contained in genome databases are often predicted using bioinformatics and are rarely verified experimentally, and so are not all accurate. Therefore, we devised a simple approach for determining translational start sites using a combination of epitope tagging and frameshift mutagenesis. This assay was used to determine the start sites of three Mycobacterium tuberculosis proteins: LexA, SigC and Rv1955. We were able to show that proteins may begin before or after the predicted site. We also found that a small, non-annotated open reading frame upstream of Rv1955 was expressed as a protein, which we have designated Rv1954A. This approach is readily applicable to any bacterial species for which plasmid transformation can be achieved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.