ArticlePloS one2008
Genetically-directed, cell type-specific sparse labeling for the analysis of neuronal morphology.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
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- Petabyte-Scale Multi-Morphometry of Single Neurons for Whole Brains.Neuroinformatics · 2022Article
- EZH2 depletion potentiates MYC degradation inhibiting neuroblastoma and small cell carcinoma tumor formation.Nature communications · 2022Article
- Cross-modal coherent registration of whole mouse brains.Nature methods · 2022Article
- Evaluation of a library of loxP variants with a wide range of recombination efficiencies by Cre.PloS one · 2022Article
- Developmental role of PHD2 in the pathogenesis of pseudohypoxic pheochromocytoma.Endocrine-related cancer · 2021Article
- Article
- Elimination of glutamatergic transmission from Hb9 interneurons does not impact treadmill locomotion.Scientific reports · 2021Article
- Whole-Brain Reconstruction of Neurons in the Ventral Pallidum Reveals Diverse Projection Patterns.Frontiers in neuroanatomy · 2021Article
- Generation of a Spiral Ganglion Neuron Degeneration Mouse Model.Frontiers in cell and developmental biology · 2021Article
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Authors and funding
4 authors.
Funding
Abstract
backgroundIn mammals, genetically-directed cell labeling technologies have not yet been applied to the morphologic analysis of neurons with very large and complex arbors, an application that requires extremely sparse labeling and that is only rendered practical by limiting the labeled population to one or a few predetermined neuronal subtypes. METHODS AND
findingsIn the present study we have addressed this application by using CreER technology to non-invasively label very small numbers of neurons so that their morphologies can be fully visualized. Four lines of IRES-CreER knock-in mice were constructed to permit labeling selectively in cholinergic or catecholaminergic neurons [choline acetyltransferase (ChAT)-IRES-CreER or tyrosine hydroxylase (TH)-IRES-CreER], predominantly in projection neurons [neurofilament light chain (NFL)-IRES-CreER], or broadly in neurons and some glia [vesicle-associated membrane protein2 (VAMP2)-IRES-CreER]. When crossed to the Z/AP reporter and exposed to 4-hydroxytamoxifen in the early postnatal period, the number of neurons expressing the human placental alkaline phosphatase reporter can be reproducibly lowered to fewer than 50 per brain. Sparse Cre-mediated recombination in ChAT-IRES-CreER;Z/AP mice shows the full axonal and dendritic arbors of individual forebrain cholinergic neurons, the first time that the complete morphologies of these very large neurons have been revealed in any species.
conclusionsSparse genetically-directed, cell type-specific neuronal labeling with IRES-creER lines should prove useful for studying a wide variety of questions in neuronal development and disease.
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