ArticleJournal of clinical microbiology2009
Utility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of Acinetobacter spp. and Pseudomonas aeruginosa.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Clinical Utility of PNA-FISH for Burn Wound Diagnostics: A Noninvasive, Culture-Independent Technique for Rapid Identification of Pathogenic Organisms in Burn Wounds.Journal of burn care & research : official publication of the American Burn Association · 2019Article
- Evaluation of FISH for Blood Cultures under Diagnostic Real-Life Conditions.European journal of microbiology & immunology · 2018Article
- Selective capture and sensitive fluorometric determination of Pseudomonas aeruginosa by using aptamer modified magnetic nanoparticles.Mikrochimica acta · 2018Article
- The Cost-Effectiveness of Rapid Diagnostic Testing for the Diagnosis of Bloodstream Infections with or without Antimicrobial Stewardship.Clinical microbiology reviews · 2018Review
- Article
- Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.BMC microbiology · 2013Article
- Bench-to-bedside review: Rapid molecular diagnostics for bloodstream infection--a new frontier?Critical care (London, England) · 2012Review
- Rapid identification of Acinetobacter spp. by fluorescence in situ hybridization (FISH) from colony and blood culture material.European journal of microbiology & immunology · 2011Article
- Molecular methods and platforms for infectious diseases testing a review of FDA-approved and cleared assays.The Journal of molecular diagnostics : JMD · 2011Review
- Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosa.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Adding mRNA to the list of spatially organized components in bacteria.Artificial DNA, PNA & XNA · 2010Article
- Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR.Annals of clinical microbiology and antimicrobials · 2010Article
- The era of molecular and other non-culture-based methods in diagnosis of sepsis.Clinical microbiology reviews · 2010Review
- A 'culture' shift: Application of molecular techniques for diagnosing polymicrobial infections.Biotechnology advancesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The utility of peptide nucleic acid fluorescence in situ hybridization (PNA FISH) for the detection of Acinetobacter spp. and Pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of ATCC strains and clinical isolates with select gram-negative rods. After testing 60 clinical isolates, PNA FISH had a sensitivity and specificity of 100% and 100%, respectively, for Acinetobacter spp. and 100% and 95%, respectively, for P. aeruginosa. PNA FISH was able to detect both pathogens simultaneously and directly from spiked blood cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.