ArticlePlant cell reports2009
Efficient, reproducible Agrobacterium-mediated transformation of sorghum using heat treatment of immature embryos.
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed.
- Efficient Embryogenic Callus Induction andPlants (Basel, Switzerland) · 2026Article
- Optimized Protocol for Sorghum Regeneration: Enhancing Embryogenic Callus Formation from Immature Inflorescences.Recent patents on biotechnology · 2026Article
- An accelerated transgene-free genome editing system using microparticle bombardment of sorghum immature embryos.aBIOTECH · 2025Article
- Sorghum as a monocot model for drought research.Frontiers in plant science · 2025Review
- Faster induction of photosynthesis increases biomass and grain yield in glasshouse-grown transgenic Sorghum bicolor overexpressing Rieske FeS.Plant biotechnology journal · 2023Article
- Leaf transformation for efficient random integration and targeted genome modification in maize and sorghum.Nature plants · 2023Article
- Highly Efficient and Reproducible Genetic Transformation in Pea for Targeted Trait Improvement.ACS agricultural science & technology · 2022Article
- Morphogene-assisted transformation of Sorghum bicolor allows more efficient genome editing.Plant biotechnology journal · 2022Article
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy.Journal of experimental botany · 2022Review
- Sorghum genetic, genomic, and breeding resources.Planta · 2021Review
- Article
- Review
- Isopentenyl Transferase (IPT) Gene Transfer to Perennial Ryegrass Through Sonication-Assisted Agrobacterium-Mediated Transformation (SAAT), Vacuum and Heat Treatment.Molecular biotechnology · 2019Article
- Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Biotechnology for biofuels · 2019Article
- Improvement ofPlant biotechnology (Tokyo, Japan) · 2019Article
- Down-Regulation ofFrontiers in nutrition · 2019Article
- An efficient and reproduciblePlant methods · 2019Article
- Progress in Optimization ofInternational journal of molecular sciences · 2018Review
- Development of a stablePlant biotechnology (Tokyo, Japan) · 2018Article
- Sweet sorghum as biofuel feedstock: recent advances and available resources.Biotechnology for biofuels · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A number of parameters related to Agrobacterium-mediated infection were tested to optimize transformation frequencies of sorghum (Sorghum bicolor L.). A plasmid with a selectable marker, phosphomannose isomerase, and an sgfp reporter gene was used. First, storing immature spikes at 4 degrees C before use decreased frequency of GFP-expressing calli, for example, in sorghum variety P898012 from 22.5% at 0 day to 6.4% at 5 days. Next, heating immature embryos (IEs) at various temperatures for 3 min prior to Agrobacterium infection increased frequencies of GFP-expressing calli, of mannose-selected calli and of transformed calli. The optimal 43 degrees C heat treatment increased transformation frequencies from 2.6% with no heat to 7.6%. Using different heating times at 43 degrees C prior to infection showed 3 min was optimal. Centrifuging IEs with no heat or heating at various temperatures decreased frequencies of all tissue responses; however, both heat and centrifugation increased de-differentiation of tissue. If IEs were cooled at 25 degrees C versus on ice after heating and prior to infection, numbers with GFP-expressing cells increased from 34.2 to 49.1%. The most optimal treatment, 43 degrees C for 3 min, cooling at 25 degrees C and no centrifugation, yielded 49.1% GFP-expressing calli and 8.3% stable transformation frequency. Transformation frequencies greater than 7% were routinely observed using similar treatments over 5 months of testing. This reproducible frequency, calculated as numbers of independent IEs producing regenerable transgenic tissues, confirmed by PCR, western and DNA hybridization analysis, divided by total numbers of IEs infected, is several-fold higher than published frequencies.
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19115059What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.