ArticleProceedings of the National Academy of Sciences of the United States of America2009
A regulatory interplay between miR-27a and Runx1 during megakaryopoiesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 126 citations in OpenAlex.
- Comprehensive analysis of microRNA expression provides mechanistic insights into transcriptomic alteration in primary and metastatic testicular germ cell tumors.Scientific reports · 2025Article
- Young extracellular vesicles restore burn-induced adipose tissue immunometabolic and mitochondrial function in older mice.Scientific reports · 2025Article
- Epigenetic regulation of megakaryopoiesis and platelet formation.Haematologica · 2024Review
- Insight into microRNAs' involvement in hematopoiesis: current standing point of findings.Stem cell research & therapy · 2023Review
- Analysis of MIR27A (rs11671784) Variant Association with Systemic Lupus Erythematous.Life (Basel, Switzerland) · 2023Article
- MiR-27a downregulates 14-3-3θ, RUNX1, AF4, and MLL-AF4, crucial drivers of blast transformation in t(4;11) leukemia cells.Cell biochemistry and function · 2022Article
- Regulation of MicroRNAs.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- Role of miRNAs and lncRNAs in hematopoietic stem cell differentiation.Non-coding RNA research · 2021Review
- Identification of a thymus microRNA‑mRNA regulatory network in Down syndrome.Molecular medicine reports · 2019Article
- Effects of somatic alterations at pathway level are more mechanism-explanatory and clinically applicable to quantity of liver metastases of colorectal cancer.Cancer medicine · 2019Article
- Identification of the Regulatory Elements and Target Genes of Megakaryopoietic Transcription Factor MEF2C.Thrombosis and haemostasis · 2019Article
- miR-30 Family miRNAs Mediate the Effect of Chronic Social Defeat Stress on Hippocampal Neurogenesis in Mouse Depression Model.Frontiers in molecular neuroscience · 2019Article
- The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation.Experimental hematology · 2018Article
- MicroRNA-9 promotes cell proliferation by regulating RUNX1 expression in human megakaryocyte development.Cell proliferation · 2018Article
- Abnormal microRNA expression in the course of hematological malignancies.Cancer management and research · 2018Review
- RUNX1 promote invasiveness in pancreatic ductal adenocarcinoma through regulating miR-93.Oncotarget · 2017Article
- Beyond mRNA: The role of non-coding RNAs in normal and aberrant hematopoiesis.Molecular genetics and metabolism · 2017Review
- The miR-23a~27a~24-2 microRNA cluster buffers transcription and signaling pathways during hematopoiesis.PLoS genetics · 2017Article
- The Role of Runx1 in Embryonic Blood Cell Formation.Advances in experimental medicine and biology · 2017Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcription factor Runx1 is a key regulator of definitive hematopoiesis in the embryo and the adult. Lineage-specific expression of Runx1 involves transcription and post-transcription control through usage of alternative promoters and diverse 3'UTR isoforms, respectively. We identified and mapped microRNA (miR) binding sites on Runx1 3'UTR and show that miR-27a, miR-9, miR-18a, miR-30c, and miR-199a* bind and post-transcriptionally attenuate expression of Runx1. miR-27a impacts on both the shortest (0.15 kb) and longest (3.8 kb) 3'UTRs and, along with additional miRs, might contribute to translation attenuation of Runx1 mRNA in the myeloid cell line 416B. Whereas levels of Runx1 mRNA in 416B and the B cell line 70Z were similar, the protein levels were not. Large amounts of Runx1 protein were found in 70Z cells, whereas only minute amounts of Runx1 protein were made in 416B cells and overexpression of Runx1 in 416B induced terminal differentiation associated with megakaryocytic markers. Induction of megakaryocytic differentiation in K562 cells by 12-o-tetradecanoylphorbol-13-acetate markedly increased miR-27a expression, concomitantly with binding of Runx1 to miR-27a regulatory region. The data indicate that miR-27a plays a regulatory role in megakaryocytic differentiation by attenuating Runx1 expression, and that, during megakaryopoiesis, Runx1 and miR-27a are engaged in a feedback loop involving positive regulation of miR-27a expression by Runx1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.