ArticleThe Plant cell2008
Functional proteomics of Arabidopsis thaliana guard cells uncovers new stomatal signaling pathways.
Article in The Plant cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 134 papers.
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Who cites it
134 citing papers in PubMed, 289 citations in OpenAlex.
- Stomatal movement as an emergent property of the remarkable plasticity of guard cells.The New phytologist · 2026Article
- Sulfur metabolism-dependent retrograde signaling for oxidative stress acclimation.Journal of experimental botany · 2026Review
- Cell-type specific early perception of nine phytohormones revealed by single-nucleus transcriptomics in Arabidopsis.Nature communications · 2026Article
- Cell-type-specific compartmentalization and function of the glucosinolate-myrosinase system in Arabidopsis thaliana.The Journal of biological chemistry · 2026Article
- Mitigation of soil water stress by moderately deep sowing and exogenous application of glucosinolates during the early seedling stage in rapeseed.Frontiers in plant science · 2026Article
- Deciphering the metabolic patterns of cashew apple ripening process: A comprehensive non-targeted metabolomics analysis.Food chemistry. Molecular sciences · 2025Article
- INTACT-based guard cell transcriptomes from a progressive drought time course reveal targets for modifying stomatal responses.The Plant cell · 2025Article
- TGG1 and TGG2 mutations impair allyl isothiocyanate-mediated stomatal closure in Arabidopsis thaliana.Protoplasma · 2025Article
- Glucosinolates can act as signals to modulate intercellular trafficking via plasmodesmata.The New phytologist · 2025Article
- A Genome-Wide Association Screen for Genes Affecting Leaf Trichome Development and Epidermal Metal Accumulation in Arabidopsis.Plant, cell & environment · 2025Article
- Opportunities and Challenges in Advancing Plant Research with Single-cell Omics.Genomics, proteomics & bioinformatics · 2024Review
- Spectral Reflectance Indexes Reveal Differences in the Physiological Status ofPlants (Basel, Switzerland) · 2023Article
- Proximity Labeling in Plants.Annual review of plant biology · 2023Review
- Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals.Nature communications · 2023Article
- Omics Approaches in Invasion Biology: Understanding Mechanisms and Impacts on Ecological Health.Plants (Basel, Switzerland) · 2023Review
- Transcriptome and proteome depth analysis indicate ABA, MAPK cascade and CaFrontiers in plant science · 2023Article
- Proteomic Profiling of Plant and Pathogen Interaction on the Leaf Epidermis.International journal of molecular sciences · 2022Article
- Non-autonomous stomatal control by pavement cell turgor via the K+ channel subunit AtKC1.The Plant cell · 2022Article
- The Cellular and Subcellular Organization of the Glucosinolate-Myrosinase System against Herbivores and Pathogens.International journal of molecular sciences · 2022Review
- Effects of Plant Hormones, Metal Ions, Salinity, Sugar, and Chemicals Pollution on Glucosinolate Biosynthesis in Cruciferous Plant.Frontiers in plant science · 2022Review
74 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We isolated a total of 3 x 10(8) guard cell protoplasts from 22,000 Arabidopsis thaliana plants and identified 1734 unique proteins using three complementary proteomic methods: protein spot identification from broad and narrow pH range two-dimensional (2D) gels, and 2D liquid chromatography-matrix assisted laser desorption/ionization multidimensional protein identification technology. This extensive single-cell-type proteome includes 336 proteins not previously represented in transcriptome analyses of guard cells and 52 proteins classified as signaling proteins by Gene Ontology analysis, of which only two have been previously assessed in the context of guard cell function. THIOGLUCOSIDE GLUCOHYDROLASE1 (TGG1), a myrosinase that catalyzes the production of toxic isothiocyanates from glucosinolates, showed striking abundance in the guard cell proteome. tgg1 mutants were hyposensitive to abscisic acid (ABA) inhibition of guard cell inward K(+) channels and stomatal opening, revealing that the glucosinolate-myrosinase system, previously identified as a defense against biotic invaders, is required for key ABA responses of guard cells. Our results also suggest a mechanism whereby exposure to abiotic stresses may enhance plant defense against subsequent biotic stressors and exemplify how enhanced knowledge of the signaling networks of a specific cell type can be gained by proteomics approaches.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.