ArticleJournal of bacteriology2009
Regulation of expression of the region 3 promoter of the Escherichia coli K5 capsule gene cluster involves H-NS, SlyA, and a large 5' untranslated region.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- ChronicInternational journal of molecular sciences · 2025Article
- RyhB Regulates Capsular Synthesis for Serum Resistance and Virulence of Avian PathogenicInternational journal of molecular sciences · 2025Article
- Article
- Regulation ofFrontiers in microbiology · 2022Review
- Article
- High density fermentation of probiotic E. coli Nissle 1917 towards heparosan production, characterization, and modification.Applied microbiology and biotechnology · 2021Article
- Three tandem promoters, together with IHF, regulate growth phase dependent expression of the Escherichia coli kps capsule gene cluster.Scientific reports · 2017Article
- Identification of new members of the Escherichia coli K-12 MG1655 SlyA regulon.Microbiology (Reading, England) · 2017Article
- Allelic Variation of the Capsule Promoter Diversifies Encapsulation and Virulence In Streptococcus pneumoniae.Scientific reports · 2016Article
- Characterization of SlyA in Shigella flexneri Identifies a Novel Role in Virulence.Infection and immunity · 2016Article
- Cyclic AMP (cAMP) Receptor Protein-cAMP Complex Regulates Heparosan Production in Escherichia coli Strain Nissle 1917.Applied and environmental microbiology · 2015Article
- Phenotypic Heterogeneity in Expression of the K1 Polysaccharide Capsule of Uropathogenic Escherichia coli and Downregulation of the Capsule Genes during Growth in Urine.Infection and immunity · 2015Article
- Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules.FEMS microbiology reviews · 2014Review
- Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB.Bioscience reports · 2013Article
- Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.The Journal of biological chemistry · 2012Article
- A potential role for glucose transporters in the evolution of human brain size.Brain, behavior and evolution · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Escherichia coli group 2 capsule gene clusters are temperature regulated, being expressed at 37 degrees C but not at 20 degrees C. Expression is regulated at the level of transcription by two convergent promoters, PR1 and PR3. In this paper, we show that regulation of transcription from PR3 involves a number of novel features including H-NS, SlyA, and a large 741-bp 5' untranslated region (UTR). H-NS represses transcription from PR3 at 20 degrees C and binds both 5' and 3' of the transcription start site. The 3' downstream regulatory element (DRE) was essential for temperature-dependent H-NS repression. At 37 degrees C, SlyA activates transcription independent of H-NS but maximal transcription requires H-NS. The UTR is present between the transcription start site and the first gene in the operon, kpsM. We demonstrate that the UTR, as well as containing the H-NS DRE, functions to moderate the extent of transcription that reaches kpsM and allows the binding of antitermination factor RfaH.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.