ArticleJournal of bacteriology2009
Characterization of the Escherichia coli O157:H7 Sakai GadE regulon.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 58 citations in OpenAlex.
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- Comparative genome analysis of enterohemorrhagic Escherichia coli ATCC 43894 and its pO157-cured strain 277.Journal of microbiology (Seoul, Korea) · 2025Article
- The type 6 secretion system immunity protein RhsFI has been repurposed for small RNA regulation in pathogenic Escherichia coli.Nucleic acids research · 2025Article
- Determinants of Antibiotic Resistance and Virulence Factors in the Genome ofAntibiotics (Basel, Switzerland) · 2024Article
- Genome engineering of Stx1-and Stx2-converting bacteriophages unveils the virulence of the dairy isolateFrontiers in microbiology · 2023Article
- Nitric Oxide InducedMicroorganisms · 2022Article
- Insights into Emergence of Antibiotic Resistance in Acid-Adapted EnterohaemorrhagicAntibiotics (Basel, Switzerland) · 2021Review
- Designing next generation recombinant protein expression platforms by modulating the cellular stress response in Escherichia coli.Microbial cell factories · 2020Article
- An EnvironmentalFrontiers in microbiology · 2020Article
- Control freaks-signals and cues governing the regulation of virulence in attaching and effacing pathogens.Biochemical Society transactions · 2019Review
- Structural and Functional Analysis of the Escherichia coli Acid-Sensing Histidine Kinase EvgS.Journal of bacteriology · 2017Article
- Regulation ofEcoSal Plus · 2017Review
- TheAIMS microbiology · 2017Article
- Global Regulator of Virulence A (GrvA) Coordinates Expression of Discrete Pathogenic Mechanisms in Enterohemorrhagic Escherichia coli through Interactions with GadW-GadE.Journal of bacteriology · 2016Article
- Transcriptomic analysis of Shiga-toxigenic bacteriophage carriage reveals a profound regulatory effect on acid resistance in Escherichia coli.Applied and environmental microbiology · 2015Article
- Decoding genome-wide GadEWX-transcriptional regulatory networks reveals multifaceted cellular responses to acid stress in Escherichia coli.Nature communications · 2015Article
- Polyamines Stimulate the Level of the σ38 Subunit (RpoS) of Escherichia coli RNA Polymerase, Resulting in the Induction of the Glutamate Decarboxylase-dependent Acid Response System via the gadE Regulon.The Journal of biological chemistry · 2015Article
- Locus of enterocyte effacement: a pathogenicity island involved in the virulence of enteropathogenic and enterohemorragic Escherichia coli subjected to a complex network of gene regulation.BioMed research international · 2015Review
- Article
- Characterization of the YdeO regulon in Escherichia coli.PloS one · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Integrating laterally acquired virulence genes into the backbone regulatory network is important for the pathogenesis of Escherichia coli O157:H7, which has captured many virulence genes through horizontal transfer during evolution. GadE is an essential transcriptional activator of the glutamate decarboxylase (GAD) system, the most efficient acid resistance (AR) mechanism in E. coli. The full contribution of GadE to the AR and virulence of E. coli O157:H7 remains largely unknown. We inactivated gadE in E. coli O157:H7 Sakai and compared global transcription profiles of the mutant with that of the wild type in the exponential and stationary phases of growth. Inactivation of gadE significantly altered the expression of 60 genes independently of the growth phase and of 122 genes in a growth phase-dependent manner. Inactivation of gadE markedly downregulated the expression of gadA, gadB, and gadC and of many acid fitness island genes. Nineteen genes encoded on the locus of enterocyte effacement (LEE), including ler, showed a significant increase in expression upon gadE inactivation. Inactivation of ler in the DeltagadE strain reversed the effect of gadE deletion on LEE expression, indicating that Ler is necessary for LEE repression by GadE. GadE is also involved in downregulation of LEE expression under conditions of moderately acidic pH. Characterization of AR of the DeltagadE strain revealed that GadE is indispensable for a functional GAD system and for survival of E. coli O157:H7 in a simulated gastric environment. Altogether, these data indicate that GadE is critical for the AR of E. coli O157:H7 and that it plays an important role in virulence by downregulating expression of LEE.
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