ArticleAnalytical chemistry2009
Fluorescent avidin-bound silver particle: a strategy for single target molecule detection on a cell membrane.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- A superior bright NIR luminescent nanoparticle preparation and indicating calcium signaling detection in cells and small animals.Cell & bioscience · 2018Article
- Applying Taguchi design and large-scale strategy for mycosynthesis of nano-silver from endophytic Trichoderma harzianum SYA.F4 and its application against phytopathogens.Scientific reports · 2017Article
- The Effects of Different Ionic Liquid Coatings and the Length of Alkyl Chain on Antimicrobial and Cytotoxic Properties of Silver Nanoparticles.Iranian endodontic journal · 2017Article
- Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates.BioNanoScience · 2013Article
- Noble metal nanoparticles for biosensing applications.Sensors (Basel, Switzerland) · 2012Review
- Target molecule imaging on tissue specimens by fluorescent metal nanoprobes.Journal of biomedical optics · 2011Article
- Chemical analysis of single cells.Analytical chemistry · 2011Review
- Direct observation of chemokine receptors 5 on T-lymphocyte cell surfaces using fluorescent metal nanoprobes 2: Approximation of CCR5 populations.Biochemical and biophysical research communications · 2011Article
- Fluorescent Metal Nanoshells: Lifetime-Tunable Molecular Probes in Fluorescent Cell Imaging.The journal of physical chemistry. C, Nanomaterials and interfaces · 2011Article
- Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation.ACS nano · 2011Article
- Direct observation to chemokine receptor 5 on T-lymphocyte cell surface using fluorescent metal nanoprobes.Biochemical and biophysical research communications · 2010Article
- Fluorescent metal nanoshell probe to detect single miRNA in lung cancer cell.Analytical chemistry · 2010Article
- Detection of CXCR4 receptors on cell surface using a fluorescent metal nanoshell.Journal of biomedical opticsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cy5-avidin conjugate-bound silver nanoparticles were prepared as a fluorescence molecular reagent for the cell imaging. Compared with the metal-free avidin conjugate, the avidin-metal complex was observed to display a stronger emission intensity, shorter lifetime, and better photostability. The avidin-metal complexes were conjugated with the biotin-sites on the surfaces of PM1 cell lines, and the cell images were recorded using scanning confocal microscopy. It was noticed that the avidin-metal complexes bound on the cell surfaces could be identified as the isolated emission spots distinct from the cellular autofluorescence. The emission intensity over the cell image was increased with an increase of the number of avidin-metal complexes on the cell surface but the lifetime was decreased. A quantitative regression curve was achieved between the amount of avidin-metal complex on the cell surface and the emission intensity or lifetime over the entire cell image. On the basis of this curve, we expect to develop an approach that can be used to quantify the amount of target molecules on the cell surfaces using the cell intensity and lifetime images at the single cell level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.