ArticlePlant molecular biology2009
Targeted transgene integration in plant cells using designed zinc finger nucleases.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 218 citations in OpenAlex.
- Hyperhydricity Syndrome in In Vitro Plants: Mechanisms, Physiology, and Control.Plants (Basel, Switzerland) · 2025Review
- Recent Advances in Tomato Gene Editing.International journal of molecular sciences · 2024Review
- Simple promotion of Cas9 and Cas12a expression improves gene targeting via an all-in-one strategy.Frontiers in plant science · 2024Article
- Beyond green and red: unlocking the genetic orchestration of tomato fruit color and pigmentation.Functional & integrative genomics · 2023Review
- CRISPR/Cas-based tools for the targeted control of plant viruses.Molecular plant pathology · 2022Review
- Expanding Gene-Editing Potential in Crop Improvement with Pangenomes.International journal of molecular sciences · 2022Review
- Gene Targeting Facilitated by Engineered Sequence-Specific Nucleases: Potential Applications for Crop Improvement.Plant & cell physiology · 2021Review
- Cellular engineering of plant cells for improved therapeutic protein production.Plant cell reports · 2021Review
- Maize transformation: history, progress, and perspectives.Molecular breeding : new strategies in plant improvement · 2021Review
- Embryo-Engineered Nonhuman Primate Models: Progress and Gap to Translational Medicine.Research (Washington, D.C.) · 2021Review
- Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines.BMC biotechnology · 2020Article
- Challenges and Perspectives in Homology-Directed Gene Targeting in Monocot Plants.Rice (New York, N.Y.) · 2019Review
- Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox.International journal of molecular sciences · 2019Review
- Development of an Agrobacterium-delivered CRISPR/Cas9 system for wheat genome editing.Plant biotechnology journal · 2019Article
- DP-BINDER: machine learning model for prediction of DNA-binding proteins by fusing evolutionary and physicochemical information.Journal of computer-aided molecular design · 2019Article
- CRISPR/Cas9-mediated targeted T-DNA integration in rice.Plant molecular biology · 2019Article
- Plant Genome Engineering for Targeted Improvement of Crop Traits.Frontiers in plant science · 2019Review
- Generation of genetically-engineered animals using engineered endonucleases.Archives of pharmacal research · 2018Review
- CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation.Nature communications · 2018Article
- Applications of New Breeding Technologies for Potato Improvement.Frontiers in plant science · 2018Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
22 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted transgene integration in plants remains a significant technical challenge for both basic and applied research. Here it is reported that designed zinc finger nucleases (ZFNs) can drive site-directed DNA integration into transgenic and native gene loci. A dimer of designed 4-finger ZFNs enabled intra-chromosomal reconstitution of a disabled gfp reporter gene and site-specific transgene integration into chromosomal reporter loci following co-transformation of tobacco cell cultures with a donor construct comprised of sequences necessary to complement a non-functional pat herbicide resistance gene. In addition, a yeast-based assay was used to identify ZFNs capable of cleaving a native endochitinase gene. Agrobacterium delivery of a Ti plasmid harboring both the ZFNs and a donor DNA construct comprising a pat herbicide resistance gene cassette flanked by short stretches of homology to the endochitinase locus yielded up to 10% targeted, homology-directed transgene integration precisely into the ZFN cleavage site. Given that ZFNs can be designed to recognize a wide range of target sequences, these data point toward a novel approach for targeted gene addition, replacement and trait stacking in plants.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.