ArticleThe Journal of biological chemistry2009
Secreted cyclophilin A, a peptidylprolyl cis-trans isomerase, mediates matrix assembly of hensin, a protein implicated in epithelial differentiation.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Identifying In Vitro Cultured Human Hepatocytes Markers with Machine Learning Methods Based on Single-Cell RNA-Seq Data.Frontiers in bioengineering and biotechnology · 2022Article
- Article
- Inorganic Polyphosphates As Storage for and Generator of Metabolic Energy in the Extracellular Matrix.Chemical reviews · 2019Review
- Peptidyl prolylCommunications biology · 2019Article
- Review
- Renal acid-base regulation: new insights from animal models.Pflugers Archiv : European journal of physiology · 2015Review
- Collecting duct intercalated cell function and regulation.Clinical journal of the American Society of Nephrology : CJASN · 2015Review
- Molecular mechanisms and regulation of urinary acidification.Comprehensive Physiology · 2014Review
- The roles of CD147 and/or cyclophilin A in kidney diseases.Mediators of inflammation · 2014Review
- Galectin-3 mediates oligomerization of secreted hensin using its carbohydrate-recognition domain.American journal of physiology. Renal physiology · 2013Article
- Molecular cloning and characterization of a cyclophilin A homologue from Schistosoma japonicum.Parasitology research · 2012Article
- Extracellular cyclophilin-A stimulates ERK1/2 phosphorylation in a cell-dependent manner but broadly stimulates nuclear factor kappa B.Cancer cell international · 2012Article
- Molecular cloning, expression, and characterization of cyclophilin A from Clonorchis sinensis.Parasitology research · 2011Article
- Deletion of hensin/DMBT1 blocks conversion of beta- to alpha-intercalated cells and induces distal renal tubular acidosis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Contribution of epithelial plasticity to renal transplantation-associated fibrosis.Transplantation proceedings · 2010Review
- Adaptation to metabolic acidosis and its recovery are associated with changes in anion exchanger distribution and expression in the cortical collecting duct.Kidney international · 2010Article
- Molecular aspects of cyclophilins mediating therapeutic actions of their ligands.Cellular and molecular life sciences : CMLS · 2010Review
- A cyclosporin derivative discriminates between extracellular and intracellular cyclophilins.Angewandte Chemie (International ed. in English) · 2010Article
- Regulated acid-base transport in the collecting duct.Pflugers Archiv : European journal of physiology · 2009Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Hensin is a rabbit ortholog of DMBT1, a multifunctional, multidomain protein implicated in the regulation of epithelial differentiation, innate immunity, and tumorigenesis. Hensin in the extracellular matrix (ECM) induced morphological changes characteristic of terminal differentiation in a clonal cell line (clone C) of rabbit kidney intercalated cells. Although hensin is secreted in monomeric and various oligomeric forms, only the polymerized ECM form is able to induce these phenotypic changes. Here we report that hensin secretion and matrix assembly were inhibited by the peptidylprolyl cis-trans isomerase (PPIase) inhibitors cyclosporin A (CsA) and a derivative of cyclosporin A with modifications in the d-Ser side chain (Cs9) but not by the calcineurin pathway inhibitor FK506. PPIase inhibition led to failure of hensin polymerization in the medium and ECM, plus the loss of apical cytoskeleton, apical microvilli, and the columnar epithelial shape of clone C cells. Cyclophilin A was produced and secreted into the media to a much greater extent than cyclophilins B and C. Our results also identified the direct CsA-sensitive interaction of cyclophilin A with hensin, suggesting that cyclophilin A is the PPIase that mediates the polymerization and matrix assembly of hensin. These results are significant because this is the first time a direct role of peptidylprolyl cis-trans isomerase activity has been implicated in the process of epithelial differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.