ArticleMethods in enzymology2008
Assays for determining poly(A) tail length and the polarity of mRNA decay in mammalian cells.
Article in Methods in enzymology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 50 citations in OpenAlex.
- Controlling In Vitro mRNA Polyadenylation by Monitoring Poly(A) Polymerase Consumption of ATP.International journal of molecular sciences · 2026Article
- Mutagenesis studies suggest a mechanism for influenza polymerase stalling during polyadenylation.Nucleic acids research · 2025Article
- Article
- Measurement of Poly A Tail Length from Drosophila Larva Brain and Cell Line.Journal of visualized experiments : JoVE · 2024Article
- Measuring the tail: Methods for poly(A) tail profiling.Wiley interdisciplinary reviews. RNA · 2023Review
- ROCK1/MLC2 inhibition induces decay of viral mRNA in BPXV infected cells.Scientific reports · 2022Article
- Review
- Transcript Isoform-Specific Estimation of Poly(A) Tail Length by Nanopore Sequencing of Native RNA.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Poly(A) tail dynamics: Measuring polyadenylation, deadenylation and poly(A) tail length.Methods in enzymology · 2021Article
- Exploring Translational Control of Maternal mRNAs in Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Phosphodiester modifications in mRNA poly(A) tail prevent deadenylation without compromising protein expression.RNA (New York, N.Y.) · 2020Article
- Transcriptome-wide stability analysis uncovers LARP4-mediated NFκB1 mRNA stabilization during T cell activation.Nucleic acids research · 2020Article
- Article
- Poly(A)-seq: A method for direct sequencing and analysis of the transcriptomic poly(A)-tails.PloS one · 2020Article
- Article
- The Human Bocavirus 1 NP1 Protein Is a Multifunctional Regulator of Viral RNA Processing.Journal of virology · 2018Article
- Antisense transcription of the myotonic dystrophy locus yields low-abundant RNAs with and without (CAG)n repeat.RNA biology · 2017Article
- Transcriptome analyses of rhesus monkey preimplantation embryos reveal a reduced capacity for DNA double-strand break repair in primate oocytes and early embryos.Genome research · 2017Article
- Interrogating the degradation pathways of unstable mRNAs with XRN1-resistant sequences.Nature communications · 2016Article
- Mariner Transposons Contain a Silencer: Possible Role of the Polycomb Repressive Complex 2.PLoS genetics · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This chapter describes several methods for measuring the length of the mRNA poly(A) tail and a novel method for measuring mRNA decay. Three methods for measuring the length of a poly(A) tail are presented: the poly(A) length assay, the ligation-mediated poly(A) test (LM-PAT), and the RNase H assay. The first two methods are PCR-based assays involving cDNA synthesis from an oligo(dT) primer. The third method involves removing the poly(A) tail from the mRNA of interest. A major obstacle to studying the enzymatic step of mammalian mRNA decay has been the inability to capture mRNA decay intermediates with structural impediments such as the poly(G) tract used in yeast. To overcome this, we combined a standard kinetic analysis of mRNA decay with a tetracycline repressor-controlled reporter with an Invader RNA assay. The Invader RNA assay is a simple, elegant assay for the quantification of mRNA. It is based on signal amplification, not target amplification, so it is less prone to artifacts than other methods for nucleic acid quantification. It is also very sensitive, able to detect attomolar levels of target mRNA. Finally, it requires only a short sequence for target recognition and quantitation. Therefore, it can be applied to determining the decay polarity of a mRNA by measuring the decay rates of different portions of that mRNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.